Learning objectives
The module “Histology and General Veterinary Embryology “makes part of the course “Histology, Embryology and Zoology”. The module has the aim to teach the student to know and understand the notions of cytology, embryology and histology, with the purpose to gain solid basic knowledge necessary for the continuation of the educational process towards the profession of veterinary medicine.
Precisely the students need to be able to:
1. Know and understand the principles of cytology, embryology, and general histology. Be able to understand and use the appropriate scientific vocabulary. Be able to critically elaborate the acquired knowledge. (knowledge and understanding)
2. Be able to use the acquired knowledge to recognize with the optical microscope different tissues and understand the different levels of structural organization (subcellular / cellular/ tissutal); to understand the dynamics of development, starting from the gametogenesis to the formation of the three germ layers; to analyze different factors that influence the variation of the fetal circulation at birth; to understand the dynamics of the formation of the fetal membranes and placenta of veterinary species (applying knowledge and understanding)
3. The students at the end of the lessons need to demonstrate the comprehension of the different topics introduced during the lectures and plenaries, and the ability to correlate the different structural levels. In particular, they need to be able to integrate and handle the acquired knowledge in multidisciplinary areas (making judgments)
4. The students need to demonstrate the ability to communicate clearly the knowledge acquired using adequate language (communication skills)
5. The student needs to demonstrate the ability to correlate, integrate and handle the gained knowledge in multidisciplinary areas. Additionally, they need to demonstrate to have developed abilities that make them autonomous in the handling of their knowledge (learning skills)
Prerequisites
Specific knowledge required by the admission test and when necessary, recovery of the educational debt in Biology.
Course unit content
The course includes theoretical lessons that take place in the classroom and theoretical-practical lessons that take place both in the histology and in the microscopy labs. The course is divided into three parts. The first part of the course dedicated to the cytology treated of the structure of eukaryotic cell. The second part give notions of general embryology from gametogenesis to germ layers formation and regarding the species of veterinary interest, covers fetal membranes, placentation, fetal circulation and changes at birth will be treated.
In the third part, that is devoted to histology, the teacher provides knowledge on structural and functional characteristics of the various tissues and on the techniques and equipment necessary for the preparation of histological slides and their analysis. In the practical lessons the student is exercised in recognition of tissues by light microscopy. For the practical activity is required the distribution of students in different groups, the number of groups is related both with the total number of students and with the labs and room capacity.
Full programme
Frontal teaching:
Cytology:
The Biological membrane and the Plasma membrane: structure.
Cytoplasmic organelles: Smooth and Rough Endoplasmatic Reticulum, Ribosomes, Golgi Apparatus, Lysosomes, Peroxisomes, Mitocondria, Cytoskeleton: Microtubules, Microfilaments, Intermediate filaments; Centrioles, Centrosome.
Interphase Nucleus: Nuclear Envelope, Nucleoplasm: Nuclear Matrix, Chromatin: Euchromatin, Heterochromatin and Nucleolus.
Intercellular and cell to matrix junctions: Occluding junctions; Adherens Junctions: Adhesion belt, Desmosomes, Hemidesmosomes and Focal adhesions; Communicating junctions. Junctional complex.
Specializations of the apical edge of plasma membrane; structural features and localizations: Microvilli, Cilia, Sterociglia.
Stages of cell cycle. Proliferation state, potency and cell differentation.
Remind of cell division: Mitosis and Meiosis. Definition of programmed cell death.
Embriology:
Spermatogenesis e Oogenesis.
From spermiogenesis to anfimixis.
Cleavage.
Gastrulation: formation of the three germ layers.
Derivatives of the embryonic germ layers.
Placenta and extraembryonic membranes in mammals of veterinary interest.
Fetal circulation and changes at birth.
Embryonic anexa in birds.
Histology:
Instruments and methods for cell and tissue observation and study.
Methods for the preparation of a histology section: sample harvest, fixation, dehydration, embedding, sectioning, staining and mounting.
Elements of histological staining.
Structural organization of organs: Pipe organ, Parenchymatous organs, Laminar organs.
Epithelium Tissue: Embryonic origin and distinctive tissue characteristics.
Lining Epithelium: Classification and localizations: Simple epithelium, Pseudostratified Epithelium, Stratified Epithelium, Sensory Epithelium, Specialized Epithelium.
Glandular Epithelium Exocrine glands: Localizations and classification relative to: number of secretory cells, disposition respect the organ wall, secretory units characteristics, ducts system characteristics, method of secretion, secetory product.
Endocrine glands: Localizations and classification relative to: number of secretory cells, structural features: Solid cell cord gland, Follicular gland, diffuse cells gland.
Connective Tissue: Embryonic origin, Cells and extracellular matrix, Localizations and classification.
Embryonic Connective Tissue.
Adult Connective Tissue:
Proper Connective Tissue:
Collagen Connective Tissue:
Loose,
Dense:
Irregular,
Regular: woven fibres, parallel fibres.
Reticular Connective Tissue.
Elastic Connective Tissue.
Pigmented Connective Tissue.
Adipose Connective Tissue: Brown, White.
Specialized Connective Tissue:
Cartilage Tissue:
Classification and localizations: Hyaline Cartilage, Fibrocartilage, Elastic Cartilage.
Features of cartilage cells and matrix.
Perichomdrium.
Bone Tissue:
Classification and localizations: Woven bone, Lamellar bone, Compact bone, Spongy bone.
Osteogenesis.
Features of bone cells and matrix; Compact and Cancellous or Spongy bone: Bone lamellae, Osteons.
Bone Tissue and Bones:
Periosteum, Endosteum,. Medullary cavità.
Bone marrow: Red, Yellow, Gelatinous.
Diaphysis > Body. Epiphyses > Extremities. Metaphyses.
Fluid Ground Substance Connective Tissue. Blood and Lymph:
Cell and plasma components.
Hematopoiesis.
Structural characteristics of:
Erythrocytes,
Leucocytes: Monoytes, T Lymphocytes, B Lymphocytes,
Granulocytes: Neutrophils, Eosinophils, Basophils,
Platelets.
Muscle Tissue: Embryonic origin and classification of muscle tissue:
Smooth Muscle Tissue,
Striated Muscle Tissue,
Striated Cardiac Muscle Tissue.
Nervous Tissue. General details on nervous tissue and nervous system.
Histogenesis of nervous tissue.
Neurons.
Neuroglial cells.
Myelin and non-myelinated nerve fibres; Nerve fibers.
Synapses.
Practical teaching:
Visit to the histological laboratory.
Technical procedure for paraffin embedded histology.
Methods to use the components of optic microscope.
Resolution, magnification, penetration depth.
Focus and observation of histologic specimen.
Identification of histologic staining.
Identification of nucleus and cytoplasm.
Identification of the Lining Epithelium tissue:
Simple squamous Epithelium.
Simple cuboidal Epithelium.
Simple columnar Epithelium.
Pseudostratified cuboidal or columnar, ciliated Epithelium.
Stratified squamous Epithelium
Stratified squamous, more or less keratinized Epithelium.
Stratified squamous, differential keratinized Epithelium.
Stratified squamous, keratinized Epithelium: Skin.
Transitional Epithelium.
Identification of the Glandular Epithelium tissue:
Acinar–branched gland (Sebaceous gland).
Coiled-tubular gland (Sudoriparous gland).
Compound tubulo-acinar, serous secreted gland.
Compound tubulo-acinar, mixed secreted gland.
Simple tubular gland.
Branched tubular gland.
Simple unicellular gland.
Labirinthic gland: liver.
Follicular gland.
Solid cell cord gland.
Identification of the Connective tissue:
Collagen Loose Connective.
Collagen Dense Irregular Connective.
Collagen Dense Regular Connective.
Hyaline Cartilage.
Spongy and Compact Bone.
Blood (Peripheral blood smear)
Identification of the Muscle tissue:
Smooth Muscle Tissue.
Striated Muscle Tissue.
Striated Cardiac Muscle Tissue.
Identification of the neuron: cell body and cellular processes.
Bibliography
In addition to the reference texts listed below, are available to students, in the special sites dedicated to them within the university homepage, accessible through identification and password, pictures of the histological slides used during the theoretical and practical activities:
-Embryology:
Reference Text:
PELAGALLI G.V., e coll.: Embriologia, Morfogenesi e Anomalie dello Sviluppo, Idelson - Gnocchi, Napoli, 2009.
Other texts:
BARBIERI M., CARINCI P.: Embriologia, Ambrosiana, Milano, 2015.
BARONE R.: Anatomia Comparata dei Mammiferi Domestici, Vol 4, Edagricole, Bologna.
DE FELICI M., e coll.: Embriologia Umana - Morfogenesi – Processi molecolari - Aspetti Clinici, Piccin, Padova, Seconda edizione, 2014.
LATSHAW W. K.: Lo sviluppo degli Animali Domestici – Embriologia ad Orientamento Clinico, Ambrosiana, Milano.
NODEN DREW M., DE LAHUNTA A.: Embriologia degli Animali Domestici – Sviluppo normale e Malformazioni congenite, Edi-ermes, Milano.
-Cytology and Histology:
Reference Text:
ADAMO S., CARINCI P., MOLINARO., SIRACUSA G., STEFANINI M., ZIPARO E.: Istologia di V. MONESI, Piccin, Padova.
Other texts:
COOPER G. M., HAUSMAN R. E.: La cellula un approccio molecolare, Piccin, Padova.
DELLMAN H.D., EURELL J.A.: Istologia e Anatomia Microscopica Veterinaria, Ed. Grasso, Bologna, 2005.
LIEBICH H.G.: Istologia e anatomia microscopica dei Mammiferi Domestici e degli Uccelli. Edizioni Piccin 2012.
-Atlases:
Reference Text:
ROSS M. H., PAWLINA W., BARNASH T. A.: Atlante di Istologia e Anatomia Microscopica, Ambrosiana, Milano, 2010.
Other atlases:
BACHA W. J., WOOD L. M.: Atlante a colori di istologia veterinaria, Delfino, Roma.
YOUNG B., LOVE J.S., STEVENS A., HEATH J. W.: Wheater – Istologia e anatomia microscopica, Elsevier Masson, Milano.
Teaching methods
The course includes theoretical lessons that take place in the classroom and theoretical-practical lessons that take place both in the histology and microscopy labs, where the students exercise to the tissues identification by light microscopy. The practical activity is organized supplying in advance to each student, through web page accessible through user and password, the work plan of the practical activity, to allow students the preparatory study. For the practical activity is required the distribution of students in different groups, the number of groups is related to the total number of students, on the lab and room capacity and allowing each student to work independently.
Assessment methods and criteria
The final exam, structured to evaluate the achievement of the objectives of the course, include a practical and an oral tests. The practical test consist on the recognition of tissues within an histological slide by the optical microscope and produce a short written with a scheme representing the observed tissue The oral examination is based on the discussion of the written scheme from the practical test and on the answers to 3 questions, one for each part of the course, randomly identified by the computer.
The exam aims to evaluate whether the student has achieved the goal of knowledge and understanding of the content and the ability to apply the knowledge gained.
The exam grade is associated with the overall assessment of the responses given by the student, weighted according to the load time of each part of the course; however, having regard to the mode of selection of questions, nowhere are permitted answers seriously insufficient (less than 4 of 10).
The examination will be completed with the second part, in the exams scheduled, with the definition of the final evaluation. Students diagnosed with learning disabilities according the law n° 170/2010, benefit the support provided to them by law.
Other information
(Continuous program)
Fluid Ground Substance Connective Tissue. Blood and Lymph:
Cell and plasma components.
Hematopoiesis.
Structural characteristics of:
Erythrocytes,
Leucocytes: Monoytes, T Lymphocytes, B Lymphocytes,
Granulocytes: Neutrophils, Eosinophils, Basophils,
Platelets.
Muscle Tissue: Embryonic origin and classification of muscle tissue:
Smooth Muscle Tissue,
Striated Muscle Tissue,
Striated Cardiac Muscle Tissue.
Nervous Tissue. General details on nervous tissue and nervous system.
Histogenesis of nervous tissue.
Neurons.
Neuroglial cells.
Myelin and non-myelinated nerve fibres; Nerve fibers.
Synapses.
Practical teaching:
Visit to the histological laboratory.
Technical procedure for paraffin embedded histology.
Methods to use the components of optic microscope.
Resolution, magnification, penetration depth.
Focus and observation of histologic specimen.
Identification of histologic staining.
Identification of nucleus and cytoplasm.
Identification of the Lining Epithelium tissue:
Simple squamous Epithelium.
Simple cuboidal Epithelium.
Simple columnar Epithelium.
Pseudostratified cuboidal or columnar, ciliated Epithelium.
Stratified squamous Epithelium
Stratified squamous, more or less keratinized Epithelium.
Stratified squamous, differential keratinized Epithelium.
Stratified squamous, keratinized Epithelium: Skin.
Transitional Epithelium.
Identification of the Glandular Epithelium tissue:
Acinar–branched gland (Sebaceous gland).
Coiled-tubular gland (Sudoriparous gland).
Compound tubulo-acinar, serous secreted gland.
Compound tubulo-acinar, mixed secreted gland.
Simple tubular gland.
Branched tubular gland.
Simple unicellular gland.
Labirinthic gland: liver.
Follicular gland.
Solid cell cord gland.
Identification of the Connective tissue:
Collagen Loose Connective.
Collagen Dense Irregular Connective.
Collagen Dense Regular Connective.
Hyaline Cartilage.
Spongy and Compact Bone.
Blood (Peripheral blood smear)
Identification of the Muscle tissue:
Smooth Muscle Tissue.
Striated Muscle Tissue.
Striated Cardiac Muscle Tissue.
Identification of the neuron: cell body and cellular processes.
