cod. 1001627

Academic year 2013/14
1° year of course - Second semester
Professor responsible for the course unit
RAMONI Roberto
integrated course unit
9 credits
hub: PARMA
course unit

Course unit structured in the following modules:

Learning objectives

The course subject is aimed at teaching knowledge and under standing of the basics of:
1 - the fundamental properties of biological molecole and macromolecules
2 - the processes of degradation and biosynthesis of the main classes of biomolecules, and of the mechanisms of their regulation.
The student will also learn to apply the acquired knowledge and understanding to topics in the field of veterinary medicine.


It is required the exam of 'Chimica propedeutica biochimica'

Course unit content

Properties of biomolecules: carbohydrates, lipids, aminoacids and proteins. Biological membranes. Structure and function of proteins. Enzyme catalysis and kinetics.
Introduction to the metabolism: coenzymes, high energy bonds, redox potentials. Catabolism of glucose. Metabolism of glycogen. Gluconeogenesis. Tricarboxilic acids cicle and related rections. Electron transport, oxidative phosphorilation and respiratory control. Photosynthesis. Lipid metabolism: Fatty acids oxidation, keton bodies, fatty acids biosynthesi, cholesterol. Metabolim of aminoacids: transamination reactions, urea cycle, PLP-dependent reactions. Nucleotides metabolism: ex-novo purines and pyrimidine biosinthesis. Ruminal fermentations.
The flow of the genetic information: Nucleic acids: DNA and RNA. Genes, chromosomes and chromatin. Duplication and maintenance of genomic DNA. DNA transcription in prokaryotic and eukaryotic cells. Regulation of gene expression in prokaryotic and eukaryotic cells. Protein synthesis. The cell cycle.
Fundamentals of molecular biology: recombinant DNA and gene cloning. Polymerase chain reaction (PCR and RT-PCR), Southern and Northern blotting
Applications of the molecular diagnostics and of the recombinant biotechnologies to the diagnosis and prevention of diseases.

Full programme

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Nelson, D.L. e Cox, M.M. 'I principi di Biochimica di Lehninger' Editore: Zanichelli

Teaching methods

Oral lessons and laboratory

Assessment methods and criteria

oral examination

Other information

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