INNOVATIVE APPROACH FOR RISK ASSESSMENT IN MICROBIOME FOOD VALUE CHAIN
cod. 1008431

Anno accademico 2023/24
2° anno di corso - Primo semestre
Docente
- Marco CANDELA
Settore scientifico disciplinare
Chimica e biotecnologia delle fermentazioni (CHIM/11)
Field
Attività formative affini o integrative
Tipologia attività formativa
Affine/Integrativa
24 ore
di attività frontali
3 crediti
sede: UNIBO
insegnamento
in INGLESE

Modulo dell'insegnamento integrato: Risk assessment of food products to human health

Obiettivi formativi

The successful student will know: (i) main goals of a metagenome study; (ii) techniques and technologies for the metagenomes assessment; (iii) role of microbiomes in food systems, food production and connections with food quality and the risk assessment.

Prerequisiti

- - -

Contenuti dell'insegnamento

1. General Introduction the study of microbiomes: metagenomics, microbial ecosystems and microbiomes, definitions, importance and applications, main technical aspects, goals for a metagenome study, types of metagenome studies, NGS sequencing, second-and third-generation NGS platforms;
2. NGS sequencing: sampling, DNA extraction from different matrices (soil, air, water and biological samples), multiplexing and library preparation, NGS sequencing reaction; marker gene 16S sequencing and shotgun metagenomics;
3. bioinformatic and biostatistics: demultiplexing, quality filtering, reads mapping, contigues reconstruction, assignment on reference databases (KEGG, COGS, greengenes and SILVA, alpha and beta diversity), multivariate statistics
4. microbiomes in food system: microbiomes of the food system actors, microbiomes circulation in food systems and connection with food quality and safety, food system microbiome for a better food, microbiomes and risks assessment in foods and food production.

Programma esteso

- - -

Bibliografia

Notes of lectures; scientific publications

Metodi didattici

Oral lectures and scientific seminaries
Teaching tools
projector, computer, instruments of microbiological laboratory

Modalità verifica apprendimento

Theoretical knowledge will be tested in a oral examination

Altre informazioni

See the website of Marco Candela, Unibo