INNOVATIVE APPROACH FOR RISK ASSESSMENT IN MICROBIOME FOOD VALUE CHAIN
cod. 1008431

Academic year 2023/24
2° year of course - First semester
Professor
Marco CANDELA
Academic discipline
Chimica e biotecnologia delle fermentazioni (CHIM/11)
Field
Attività formative affini o integrative
Type of training activity
Related/supplementary
24 hours
of face-to-face activities
3 credits
hub: UNIBO
course unit
in ENGLISH

Integrated course unit module: Risk assessment of food products to human health

Learning objectives

The successful student will know: (i) main goals of a metagenome study; (ii) techniques and technologies for the metagenomes assessment; (iii) role of microbiomes in food systems, food production and connections with food quality and the risk assessment.

Prerequisites

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Course unit content

1. General Introduction the study of microbiomes: metagenomics, microbial ecosystems and microbiomes, definitions, importance and applications, main technical aspects, goals for a metagenome study, types of metagenome studies, NGS sequencing, second-and third-generation NGS platforms;
2. NGS sequencing: sampling, DNA extraction from different matrices (soil, air, water and biological samples), multiplexing and library preparation, NGS sequencing reaction; marker gene 16S sequencing and shotgun metagenomics;
3. bioinformatic and biostatistics: demultiplexing, quality filtering, reads mapping, contigues reconstruction, assignment on reference databases (KEGG, COGS, greengenes and SILVA, alpha and beta diversity), multivariate statistics
4. microbiomes in food system: microbiomes of the food system actors, microbiomes circulation in food systems and connection with food quality and safety, food system microbiome for a better food, microbiomes and risks assessment in foods and food production.

Full programme

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Bibliography

Notes of lectures; scientific publications

Teaching methods

Oral lectures and scientific seminaries
Teaching tools
projector, computer, instruments of microbiological laboratory

Assessment methods and criteria

Theoretical knowledge will be tested in a oral examination

Other information

See the website of Marco Candela, Unibo

2030 agenda goals for sustainable development

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