Learning objectives
The teaching Lab. Techniques of microbiological analysis aims to enable the student to autonomously draw conclusions regarding the microbiological analysis of raw materials for agro-food in accordance with what is defined in the specific objectives of the professionalizing study course. Quality and Procurement of Raw Materials for the Agro-Food and Food Microbiology Area.
In particular, the student must be able to understand the meaning of a direct and indirect microbiological analysis and express the result in the correct way.
The expected learning outcomes are:
Knowledge and understanding: Knowledge of the analytical parameters to be determined and of the methods to be applied for both qualitative and quantitative microbiological analysis of a food matrix
Application skills: apply methodologies and techniques for the microbiological analysis of food
Autonomy of judgment: to evaluate the microbiological quality of a food based on the result of a microbial count of raw materials
Communication skills: communication of a result of a microbiological analysis of a liquid and solid food.
Ability to learn: Ability to critically consult databases and websites relating to microbial counting methods for specific microorganis
Prerequisites
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Course unit content
The course completes the contents of Microbiology of raw materials and concerns the application of microbiological analysis techniques of raw materials for the agro-food industry. They are 3 CFU of exercises that include 45 hours of lessons divided between classroom and laboratory exercises.
The first part of the exercises foresee the explanation and the meaning of the different microbiological counting techniques. Then the practical application part in the laboratory is foreseen. Attendance of the hours of laboratory exercises is mandatory, unless there is a justified justification assessed by the teaching teacher. The teacher will communicate to the students, at the beginning of the lessons, the maximum number of absences allowed. The assessment of attendance takes place according to methods and criteria established by the individual teacher who makes them known to the students at the beginning of the lessons
Full programme
The purpose of the microbiological analysis of food.
Direct and indirect methods of microbiological counting
The decimal dilution
The soils of microbiology counts
He counts it in the plate
Expression of the result of a microbiological analysis of a raw material for agro-food
Principles of microscopy applied to the microbiological analysis of food
Online research of the microbiological characteristics of foods and methods of counting for the different microorganisms in different foods
Bibliography
Ann Elizabeth Vaughan Pietro Buzzini Francesca Clementi. Laboratorio didattico di microbiologia (2008) Casa Editrice Ambrosiana. Distribuzione esclusiva Zanichelli. ISBN: 9788808183224
Teaching methods
The teaching will be through frontal exercises both in the classroom and in the laboratory. The lessons will be held with the help of slides that will represent didactic material, in addition to the recommended texts. The slides will be available online on the Elly SAF website in pdf format for students and some useful videos for viewing the operational sequences of the plate count analysis. During the lessons, the appropriate use of technical language will be reiterated, and the links between the various parts of the course will be emphasized.
The content of the tutorial lessons, carried out both in the classroom and in the laboratory, individually and in groups, will be an integral part of the final evaluation.
Assessment methods and criteria
The content of this part of the course is an integral part of the assessment of learning assessment assessed for "Microbiology of raw materials"
In particular, 10 of the 31 questions required by the written test relate to this part of the teaching. These questions relate both to the concepts underlying the methods of analysis, and to the application of the microbial count formula (weighted average formula) and the correct expression of the result.
Each correct answer is worth 1 point.
Other information
attendance is compulsory
