MOLECULAR DIAGNOSTICS
cod. 1009172

Academic year 2022/23
1° year of course - First semester
Professor
- Davide FERRARI
Academic discipline
Biochimica clinica e biologia molecolare clinica (BIO/12)
Field
A scelta dello studente
Type of training activity
Student's choice
31 hours
of face-to-face activities
3 credits
hub: PARMA
course unit
in ITALIAN

Learning objectives

Defining basic terminology and describes concepts in molecular diagnostics.
Apply knowledge of cellular structure and function, especially DNA and RNA, to molecular diagnostic procedures.
Gain a solid foundation in the most commonly utilized molecular diagnostic testing protocols.
Gain thinking and analysis skills to understand new diagnostic methods.
Ability to collect information to develop a new diagnostic kit.

Prerequisites

Students should have a solid background in Molecular Biology and Recombinant DNA technology

Course unit content

1. Introduction to Molecular Diagnostics
• Impact of Human Genome Project on Molecular Diagnostics
• Molecular Diagnosis in the Post Genomic Era
• Forensic Medicine and Forensic Sciences
2. Protein drugs
3. Biomarkers
- Diagnosis of infectious diseases
- Genetic identification
- Diagnosis of genetic diseases
- Molecular diagnostics in oncology
4. Detection of protein biomarkers
- Immunoassays
- (Nucleic acid) Aptamers
5. Detection of nucleic acid biomarkers
- PCR - based methods
- DNA fingerprinting
- Other amplification methods
- Single-Strand Conformation Polymorphism
- In situ hybridisation
6. Genes reporters
7. Biosensors

Full programme

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Bibliography

Molecular Diagnostics: Promises and Possibilities (Springer). Debnath · Prasad · Bisen
Specialized articles, slides and presentations of specialists and companies, available to the students in electronic format.

Teaching methods

The course is organized in lectures that provide a theoretical basis, flanked with scientific paper discussion.

Assessment methods and criteria

Oral exam

Other information

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