HISTOPATHOLOGICAL TECHNIQUES
cod. 1005375

Academic year 2022/23
2° year of course - Second semester
Professor
LAGRASTA Costanza Anna Maria
Academic discipline
Anatomia patologica (MED/08)
Field
A scelta dello studente
Type of training activity
Student's choice
14 hours
of face-to-face activities
2 credits
hub: PARMA
course unit
in ITALIAN

Integrated course unit module: BIOTECHNOLOGIES APPLIED TO DIAGNOSTIC

Learning objectives

This course will lead the students to develop knowledge and understanding of the basic principles for the correct handling and processing of histologic and cytologic samples. Moreover, thorough knowledge and understanding of the basis of the morphologic, immunohistochemical and molecular biology techniques ancillary to pathologic diagnosis will be reached. In the laboratories the students will apply the acquired theoretical knowledge.
The students will also acquire:
- learning skills to follow the evolution of the technological and instrumental progress in the pathology field, to understand and, in case, contribute to the introduction of new technical advances. This may be especially relevant for the molecular biology techniques that are becoming more and more essential in the field of oncologic diagnosis and therapy.

Prerequisites

Knowledge of the basis of histology and cellular biology, microscopy.

Course unit content

The first lesson of the course will focus on the procedures that characterize a histopathology laboratory, including its organization, software management system, instrumentation and specific measures for safety and damage prevention. The technical FISH, cells culture and immunohistochemistry will be fully explored.
The second part of the course will be dedicated to laboratories activities of the technicians over descripted.
The last part of the course will focus on the observation of the obtained samples by fluorescence microscopy

Full programme

Immunohistochemistry: principles and applications to histopathologic diagnosis
Preparation of cytological specimens: smear,, cytocentrifugation; fixation, staining
Cell cultures
Molecular biology techniques applied to Pathologic Anatomy:
- FISH (Fluorescent In Situ Hybridization): general principles, applications to oncologic pathology (HER2 and Met gene amplification, ALK and ROS-1 gene translocation, 1p19q deletion);
Special stains in histopathology: practical applications
Immunohistochemistry: practical applications

Bibliography

AA.VV. Anatomia Patologica: le basi. A cura di L. Ruco, A. Scarpa. UTET Scienze Mediche, Torino, 2007
AA.VV. Manuale di Tecnica Cito-Istologica. A cura di S. Daniel, T. Zanin.
Documentazione Scientifica Editrice (DSE), Bologna, 1997
PDF files PPT slides presented during the course

Teaching methods

The course will be based on frontal teaching: attendance to at least 70% of the classes will be mandatory. At the end of each lesson, the PDF file of the Power Point slides will be uploaded and published online in the dedicated
university webpage (http://elly.medicina.unipr.it), representing the main didactic material for the exam preparation
There will be formal lectures, interactive activities, internship and additional didactics.
Depending on the legislative provisions in force, it will be possible that the course is delivered as lectures or remotely.

Assessment methods and criteria

Multiple-choice test.
In the event of emergencies of any nature that require learners and teachers to be far away from the teaching structures, the exam sessions will be completed online on the TEAMS Office 365 platform.

Other information