Learning objectives
Defining basic terminology and describes concepts in molecular diagnostics.
Apply knowledge of cellular structure and function, especially DNA and RNA, to molecular diagnostic procedures.
Gain a solid foundation in the most commonly utilized molecular diagnostic testing protocols.
Gain thinking and analysis skills to understand new diagnostic methods.
Ability to collect information to develop a new diagnostic kit.
Prerequisites
Students should have a solid background in Molecular Biology and Recombinant DNA technology
Course unit content
1. Introduction to Molecular Diagnostics
• Impact of Human Genome Project on Molecular Diagnostics
• Molecular Diagnosis in the Post Genomic Era
• Forensic Medicine and Forensic Sciences
2. Protein drugs
3. Biomarkers
- Diagnosis of infectious diseases
- Genetic identification
- Diagnosis of genetic diseases
- Molecular diagnostics in oncology
4. Detection of protein biomarkers
- Immunoassays
- (Nucleic acid) Aptamers
5. Detection of nucleic acid biomarkers
- PCR - based methods
- DNA fingerprinting
- Other amplification methods
- Single-Strand Conformation Polymorphism
- In situ hybridisation
6. Genes reporters
7. Biosensors
Full programme
- - -
Bibliography
Molecular Diagnostics: Promises and Possibilities (Springer). Debnath · Prasad · Bisen
Specialized articles, slides and presentations of specialists and companies, available to the students in electronic format.
Teaching methods
The course is organized in lectures that provide a theoretical basis, flanked with scientific paper discussion.
Assessment methods and criteria
Oral exam
Other information
- - -
