cod. 1006769

Academic year 2018/19
1° year of course - Second semester
Academic discipline
Biologia molecolare (BIO/11)
A scelta dello studente
Type of training activity
Student's choice
48 hours
of face-to-face activities
6 credits
hub: PARMA
course unit

Learning objectives

Describe rational and combinatorial methods of protein engineering.
Learn specific examples of engineered proteins and their applications.
Gain the skills to design a basic protein engineering experiment.
Defining basic terminology and describes concepts in molecular diagnostics.
Apply knowledge of cellular structure and function, especially DNA and RNA, to molecular diagnostic procedures.
Gain a solid foundation in the most commonly utilized molecular diagnostic testing protocols.
Gain thinking and analysis skills to understand new diagnostic methods.
Ability to collect information to develop a new diagnostic kit.


Students should have a solid background in Molecular Biology and Recombinant DNA technology

Course unit content

1. Introduction to Protein Engineering and Molecular Diagnostics
2. Methods
2.1. Recombinant Protein Technology
2.2. Protein Evolution: Cell surface and phage display technologies
2.3. Cell-free protein engineering technologies
2.4. Library creation and screening/selection
2.5. Proteins as reporters
2.6. Antibodies and Therapeutic protein engineering
2.7. Aptamers: In Vitro DNA Selection
2.8. Immunoassays
2.9. Nucleic Acid Hybridization techniques (in situ, FISH, Microarray, etc.)

3. Applications
3.1. DNA Biosensors
3.2. Molecular Microbiological Testing
3.3. Biomarkers
3.4. Impact of Human Genome Project on Molecular Diagnostics
3.5. Molecular Diagnosis in the Post Genomic Era
3.6. Diagnosis and Monitoring of Infections
3.7. Diagnosis of Mutation and Genetic Disorders
3.8. Diagnosis of Complex Diseases
3.9. Forensic Medicine and Forensic Sciences

Full programme

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Molecular Diagnostics: Promises and Possibilities (Springer). Debnath · Prasad · Bisen
Specialized articles, slides and presentations of specialists and companies, available to the students in electronic format

Teaching methods

The course is organized in lectures that provide a theoretical basis, flanked with scientific paper discussion and exercices on experimental design.

Assessment methods and criteria

Oral exam

Other information

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