LABORATORY OF TIME RESOLVED SPECTROSCOPIES
cod. 1006214

Academic year 2016/17
1° year of course - Second semester
Professor
Academic discipline
Fisica applicata (a beni culturali, ambientali, biologia e medicina) (FIS/07)
Field
Sperimentale applicativo
Type of training activity
Characterising
62 hours
of face-to-face activities
6 credits
hub: PARMA
course unit
in - - -

Learning objectives

This course intends to explore the foundations of selected spectroscopic methods normally used to investigate molecular properties. Ground and excited state properties as well as their interactions with the environment will be dealt with, with steady state and time resolved methodologies. The spectroscopic techniques dealt with in this course are endowed with time resolution which is adequate for studying transient or unstable molecular states.

Students will learn how to interpret the significance of experimental results emerging from the optical methods covered in the course and become capable of performing experiments using the methods covered with the laboratory practice.

knowledge and understanding
After attending this course, students will become familiar with the techniques covered by the contents (see program)

applying knowledge and understanding
Student will be able to identify what is the specific methodology which is suitable in the study of a specific molecular property.
Students will be able to understand the meaning of an experimental result obtained with the methodologies they have learned during the course.
Students will be able to perform experiments on their own with the techniques subject of this course

making judgements
At the end of this course, students will have acquired capability of making judgments about the choice of the specific methodology, the use of the technique and the analysis of the collected data.
Students will have to draw conclusions on the molecular events that have been studied and identify unclear aspects or incomplete description in order to plan future complementary experiments.

learning skills
Understanding of the underlying principles and the critical application of the methods will enable students to expand their capabilities to understand and use correlated methodologies.

Prerequisites

Electromagnetism, classical geometrical and wave optics, quantum mechanics, condensed matter physics

Course unit content

Radiation-Matter interactions
Time resolved optical methods
Excited state processes
Fluorescence spectroscopy methods
Mechanisms and dynamics of competitive de-excitation
Photothermal spectroscopies
Single molecule detection
Laboratory practice

Full programme

Radiation-Matter interactions
Steady state optical methods
UV-vis Absorption spectroscopy
Polarization, anisotropy
Time resolved optical methods
Time resolved absorption
Pump and probe methods
Laser flash photolysis
Excited state processes
Fluorescence spectroscopy methods
Time-Correlated Single-Photon Counting
Laser sources and detection
Frequency-Domain detection
Mechanisms and dynamics of competitive de-excitation
Photothermal spectroscopies
Photoacoustics
Thermal lensing and grating
Single molecule detection
Fluorescence Correlation Spectroscopy
Single molecule FRET

Laboratory practice

Bibliography

Principles of Fluorescence Spectroscopy, Third Edition, Joseph R. Lakowicz, Springer
Original research papers and reviews published on scientific journals

Teaching methods

The course consists in a series of lectures covering the fundamentals of the spectroscopic methodologies. For those methods available at this Department, laboratory practice will be taken.

Assessment methods and criteria

Student reports on the practice and the methods introduced in the course

Other information

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