cod. 1001299

Academic year 2009/10
1° year of course - Second semester
Academic discipline
Fisica sperimentale (FIS/01)
Sperimentale applicativo
Type of training activity
48 hours
of face-to-face activities
6 credits
hub: -
course unit
in - - -

Integrated course unit module: LABORATORY 1

Learning objectives

This course will offer an overview o the methodologies based on the fluorescence emission by organic molecules useful for the characterization of functional a structural properties of biological macromolecules<br />
The aim of this course is to introduce qualitatively experimental methodologies and discuss their potential and their applicability.<br />
Laboratory experiences will allow students to apply directly the concepts outlined during the introductory part.


Mathematics and Physics (laurea triennale). Basic concepts of optical absorbance spectroscopy.<br />
Basic knowledge of photophysical processes in organic molecules

Course unit content

 Introduction<br />
Molecular photophysical processes<br />
Radiative processes<br />
Non radiative processes<br />
Intermolecular interactions<br />
Jablonski diagram<br />
Fluorescence<br />
Lifetime<br />
Quantum yield<br />
Decay kinetics<br />
Excitation and emissione spctra<br />
Solvent effect on the emission spectra<br />
Polarization<br />
Interactions with solutes<br />
FRET<br />
Steady state and time resolved methods<br />
<br />
<strong>Laboratory</strong><br />
Excitation and emission spctra of organic compounds<br />
Fluorescence quenching and intermolecular interactions (Stern Volmer)<br />
Fluorescence emission by aromatic amino acids<br />
FRET inside proteins<br />
Fluorescence emission by labeled proteins<br />
Emission spectra by Green fluorescent protein (GFP)<br />
Binding of organic dyes to proteins

Full programme

- - -


La luminescenza. Elementi di fotofisica molecolare<br />
Ivan Baraldi<br />
Bononia University Press

Teaching methods

- - -

Assessment methods and criteria

- - -

Other information

- - -