MEDICAL, VETERINARY ANF PHARMACEUTICAL BIOTECHNOLOGIES

Professor: Roberto SALA
Academic discipline: Patologia generale (MED/04)
Field: Discipline biotecnologiche comuni
Type of training activity: Characterising

24 hours
of face-to-face activities

3 credits

hub: -

course unit
in - - -

lectures timetable unavailable

exam calls

Integrated course unit module: IN VIVO AND IN VITRO TESTING MODELS

Learning objectives

This core module aims to introduce students to the concepts of ex vivo ed in vitro cell models used to study pathological processes involved in the major diseases.

Prerequisites

None

Course unit content

-Organ and tissue culture
-Extracellular matrix and tissue dissociation
-Cell purification
-Cell lines
-Cell cloning
-Culture of animal cells
-Culture contamination
-Gene transfer
-Cell vitality and cell proliferation assays
-Citotoxicity assays
-Organotypic culture models

Full programme

Strategy of cell cultures
Biology of cultured cells.
Advantages and disadvantages of cell culture.
The environment of the cell culture: substrates, media, temperature, growth factors, etc.. "Scaling-up of cell cultures.
Kinetics of growth. Survival and toxicity.
Tissue dissociation and primary cultures.
Separation and characterization of specific cell types.
Maintenance of cultures.
Cryopreservation
Contamination of cell cultures.
The cancer cell: morphological and metabolic features.
Established cell lines.
Methods for transferring therapeutic genes
• transfection by physical methods: microinjection, electroporation, bombardment (gene gun)
• Lipofezione: liposomes, cationic lipid-DNA complexes
• viral vectors: retrovirus, adenovirus, adeno-associated virus, human herpesvirus.

Bibliography

R. Ian Freshney, Culture of Animal Cells: A Manual of Basic Technique, 5th Edition, ISBN: 0-471-45329-3 Ed Wiley-
Jan-Thorsten Schantz & Kee Woei Ng: A MANUAL FOR PRIMARY HUMAN CELL CULTURE - ISBN 981-238-796-X(pbk) ed. World Scientific
Alan Doyle , J. Bryan Griffiths: Cell and Tissue Culture: Laboratory Procedures in Biotechnology ISBN: 0-471-98255-5- Ed Wiley

Teaching methods

Interactive oral lesson. Iconographic support will be provided.

Assessment methods and criteria

Oral examination

Other information

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2030 agenda goals for sustainable development

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Contacts

Toll-free number

800 904 084

Segreteria studenti

E. [segreteria.medicina@unipr.it]
T. +39 0521 903700

Servizio per la qualità della didattica

Manager della didattica:
Rag. Sabrina Ferrari
T. +39 0521 903109
E. servizio [didattica.dimec @unipr.it]

Presidente del corso di studio

Prof, Nicola Giuliani
E. nicola.giuliani@unipr.it

Delegato orientamento in uscita

Prof.ssa Sara Nicoli
E.sara.nicoli@unipr.it

Delegati Erasmus

Prof. Roberto Sala

E. roberto.sala@unipr.it

Referente assicurazione qualità

Prof.ssa Carlotta Compari
E. carlotta.compari@unipr.it

Tirocini formativi

Prof.ssa Laura Giovati

E. laura.giovati@unipr.it

GMO AND ANIMAL CELLULAR MODELS cod. 1002430