GENETICS AND INTEGRATED BIOTECHNOLOGIES LABORATORY I
cod. 1000863

Academic year 2024/25
1° year of course - Second semester
Professor
Cristina DALLABONA
Academic discipline
Genetica (BIO/18)
Field
Discipline biotecnologiche comuni
Type of training activity
Characterising
109 hours
of face-to-face activities
9 credits
hub: PARMA
course unit
in ITALIAN

Learning objectives


To provide the first year students with the basic concepts of classical and molecular genetics

Prerequisites


None

Course unit content


The cours is composed of two main sections.

1.CLASSICAL GENETICS: Mitosis and meiosis. Mendelian genetics. Independent assortment and genetic characters segregation. Modification of mendelian ratios: Co-dominance, Multiple alleles, Lethal alleles, Gene interaction. X-linked genes. Chromosome theory of inheritance. Linkage and recombination. Crossing over. Recombination in yeast (tetrads analysis). Mechanisms of genetic recombination in prokaryotes: bacterial transformation, bacterial conjugation, transduction

2. MOLECULAR BASIS OF HEREDITY: Experiments leading to the discovery of DNA as genetic material. DNA: structure and replcation. Chromosome structure in prokaryotes and eukaryotes. RNA structure, transcription and translation. The genetic code and its deciphering. Gene structure and organisation. Interrupted genes. The one-gene: one-enzyme hypothesis. Dissection of biochemical pathways. Genetic complementation. Gene expression. Genome, chromosomes and gene mutations. Molecular basis of the mutation. Reversion. Suppression. Detection of spontaneous or induced mutants. Regulation of gene expression in prokaryotes. Operon Lac: negative and positive control. Operon TRP. Retroinibition

Practical and theoric activities are supporting the lessons.

Full programme


1.CLASSICAL GENETICS: Mitosis and meiosis. Mendelian genetics. Independent assortment and genetic characters segregation. Modification of mendelian ratios: Co-dominance, Multiple alleles, Lethal alleles, Gene interaction. X-linked genes. Chromosome theory of inheritance. Linkage and recombination. Crossing over. Recombination in yeast (tetrads analysis). Mechanisms of genetic recombination in prokaryotes: bacterial transformation, bacterial conjugation, transduction
2. MOLECULAR BASIS OF HEREDITY: Experiments leading to the discovery of DNA as genetic material. DNA: structure and replcation. Chromosome structure in prokaryotes and eukaryotes. RNA structure, transcription and translation. The genetic code and its deciphering. Gene structure and organisation. Interrupted genes. The one-gene: one-enzyme hypothesis. Dissection of biochemical pathways. Genetic complementation. Gene expression. Genome, chromosomes and gene mutations. Molecular basis of the mutation. Reversion. Suppression. Detection of spontaneous or induced mutants. Regulation of gene expression in prokaryotes. Operon Lac: negative and positive control. Operon TRP. Retroinibition

Practical and theoric activities are supporting the lessons.

Bibliography


Snustad DP, Simmons MJ (2014)
Principi di Genetica
5a Edizione
EdiSES

Binelli, Ghisotti (2018)
Genetica
EdiSES

Griffiths AJF, Wessler SR, Carroll SB, Doebley J (2013)
Genetica
7a Edizione
Zanichelli Ed

Teaching methods


Frontal lectures and exercises; laboratory activities

Assessment methods and criteria


Written and oral exam

Other information

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