Vai al contenuto principale Vai al footer
Università degli Studi di Parma Università degli Studi di Parma
Degree course in
Biotechnology
Università degli Studi di Parma
Department of Chemistry, Life Sciences and Environmental Sustainability

GENETIC ENGINEERING OF PLANTS AND ANIMALS
cod. 1006123

Academic year 2022/23
2° year of course - Second semester
Professor
- Marina CALDARA - Elena MAESTRI
Academic discipline
Biologia applicata (BIO/13)
Field
Discipline biotecnologiche con finalità specifiche: biologiche e industriali
Type of training activity
Characterising
52 hours
of face-to-face activities
6 credits
hub: PARMA
course unit
in ITALIAN
lectures timetable unavailable
exam calls
Print

Learning objectives

The aim of the course is to illustrate the basic methods of recombinant technologies and their applications in plants and animals.

Prerequisites

- - -

Course unit content

Cloning in yeasts and fungi

Cloning in plants

Cloning vectors for plant organisms – Transformation methods for plant organisms - Obtainment of transgenic plants

Cloning in animal cells and applications in health field

Growth and properties of animal cells in culture - Selection systems for animal cells - Gene transfer into animal cells - Plasmid vectors for animal cells, amplicons and expression vectors - The fate of transferred DNA - Viral vectors and retroviral vectors for animal cells. Gene therapy: principles and methods - Experimental models – Some experimental results - Potential human models

Gene therapy: tools and main aspects

Full programme

- - -

Bibliography

- Gene Cloning and DNA Analysis: An Introduction, 7th Edition. Terry A. Brown. Zanichelli
- La scienza del DNA. Corso introduttivo sulle tecnologie del DNA ricombinante con esercitazioni di laboratorio. D.A. Milkos e G.A. Freyer. Piccin
- Ingegneria genetica. Principi e tecniche. S. Primrose et al. Zanichelli
- Ingegneria genetica. S.M. Kingsman, A.J. Kingsman. Piccin
- Molecular biotechnology: principles and applications of recombinant DNA. Bernard R. Glick
- Genetica generale e umana (volume secondo). L. De Carli, E. Boncinelli, G.A. Danieli, L. Larizza. Piccin

T.A. Brown - Biotecnologie molecolari - Zanichelli

Primrose, Twyman - Principles of Gene Manipulation and Genomics - Wiley-Blackwell

Strachan, Read - Genetica molecolare umana - Zanichelli

Glick, Patten - Molecular Biotechnology: Principles and Applications of Recombinant DNA - ASM Books

Teaching methods

The theoretical classes are integrated with seminars and practical training in laboratory.

Assessment methods and criteria

The oral examination verifies the knowledge acquired by the student and the communication skills. The written exam and the laboratory report verifies knowledge and application of knowledge. In all cases, the learning ability is verified.

Other information

- - -