cod. 1006123

Academic year 2021/22
2° year of course - Second semester
- Marina CALDARA - Elena MAESTRI
Academic discipline
Biologia applicata (BIO/13)
Discipline biotecnologiche con finalità specifiche: biologiche e industriali
Type of training activity
52 hours
of face-to-face activities
6 credits
hub: PARMA
course unit

Learning objectives

The aim of the course is to illustrate the basic methods of recombinant technologies and their applications in plants and animals.


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Course unit content

Cloning in plants

Cloning vectors for plant organisms – Transformation methods for plant organisms - Obtainment of transgenic plants

Cloning in animal cells and applications in health sector

Growth and properties of animal cells in culture - Selection systems for animal cells - Gene transfer into animal cells - Plasmid vectors for animal cells, amplicons and expression vectors - The fate of transferred DNA - Viral vectors and retroviral vectors for animal cells. Gene therapy: principles and methods - Experimental models – Some experimental results - Potential human models

Full programme

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- Gene Cloning and DNA Analysis: An Introduction, 7th Edition. Terry A. Brown. Zanichelli
- La scienza del DNA. Corso introduttivo sulle tecnologie del DNA ricombinante con esercitazioni di laboratorio. D.A. Milkos e G.A. Freyer. Piccin
- Ingegneria genetica. Principi e tecniche. S. Primrose et al. Zanichelli
- Ingegneria genetica. S.M. Kingsman, A.J. Kingsman. Piccin
- Molecular biotechnology: principles and applications of recombinant DNA. Bernard R. Glick
- Genetica generale e umana (volume secondo). L. De Carli, E. Boncinelli, G.A. Danieli, L. Larizza. Piccin

Teaching methods

The theoretical classes are integrated with seminars and practical training in laboratory.

Assessment methods and criteria

The oral examination verifies the knowledge acquired by the student and the communication skills. The written exam and the laboratory report verifies knowledge and application of knowledge. In all cases, the learning ability is verified.

Other information

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