Participation in the training activities provided (lectures and practical laboratory exercises) will allow students of the Course of Studies in Biological Sciences to acquire the general knowledge and fundamental notions for the culture of animal cells.
The course aims to describe the basic principles of cell cultures and their use in many fields of biomedical experimentation and diagnostics.
The course includes a theoretical part that illustrates:
1. Advantages and disadvantages of animal cell cultures compared to animal experiments
2. Obtaining and propagation of cell lines: primary and stabilized cell cultures; engineered cell lines.
3. Types of cell cultures: mono and bi- and three-dimensional co-cultures of cells in suspension and in adhesion.
4. Cellular models used in the field of biological and medical technologies
5. Architecture of a cell culture laboratory and safety standards:
i) Correct use of biological hoods, incubators, centrifuges, pipettes and pipettors, reagents and materials for cell culture.
ii) Contamination of cell cultures; The biological risk associated with the manipulation of human cells and the fundamental biohazard procedures to be used in the laboratory; maintenance, sterilization and waste disposal.
iii) Propagation of cell cultures and cell growth kinetics. Cell count. Cell adhesion and trypsinization. Freezing and thawing. Methods for evaluating cell proliferation, survival and toxicity. Vitality test.
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