Learning objectives
The overall objective is to provide students with the introductory concepts, terms, and tools necessary to identify, describe, and analyse microbes, microbial interactions, and microbial processes as they might occur in environment.
Prerequisites
Students need the basic notions of chemistry, organic chemistry and biochemistry that have gained at high school. For students with particular difficulties, the teacher will prepare a summary chapter that will be available on the Elly platform
Course unit content
The course will cover eukaryotic and prokaryotic microbes and viruses, but will emphasize bacteria. It will also provide a conceptual background in microbiology sufficient to enable students to take more advanced courses in related fields.
It has been designed to make the students familiar with the science of microbiology and its significance in everyday life.
Full programme
General Microbiology module
Microbiological techniques: light and electron microscopy, simple and differential staining procedures. Structure of the microbial cells at different evolution levels (Eukarya, Bacteria, Archaea). Comparative analysis and description of structures and functions of procaryotic and eukaryotic cells. Microbial nutrition: common nutritional requirments, growth factors, nutrient uptake, culture media, sterilization, pure cultures. Microbial growth: influence of aerobiosis,anaerobiosis, temperature, pH etc. on microbial growth. Microbial population growth , methods for growth measurement, continuous coltures (chemostat and turbidostat). Microbial metabolism and metabolic diversity: energy production, aerobic and anaerobic respiration, fermentation, oxigenic and anoxigenic photosynthesis, chemolithoautotrophy. Biosynthesis. Metabolism regulation at transcriptional level, translational level, feed-back inhibition. Microbial genetics: bacterial genome, mutation and mutants, genetic recombination in prokaryotes (transformation, conjugation, transduction). Plasmids and transposable elements. Microbial evolution and systematics. Prokaryotic diversity (Bacteria and Archaea), Fungi, Algae, Protista and Viruses
Environmental Microbiology Module
Metabolic diversity of microorganisms. Sampling methods, quantification and classification of environmental important microorganisms.
Classical and molecular methods for studying microbial populations (DGGE, TGGE, ARISA, T-RFLP ARDRA etc). Microbial community dynamics (colonization, successions). Introduction to genomics and metagenomics
ENVIRONMENTAL MICROBIOLOGY
Introduction to molecular methods of identification of bacteria and characterization of microbial communities.
Development and dynamics of microbial communities (colonization, primary and secondary microbial successions, etc)
Notes on the interactions between microorganisms and higher organisms: Plants (-phylosphere, rhizosphere, -micorrhizae, -nitrogen fixation, -plant pathogenic) Animals (- the rumen, - mutualistic associations between invertebrates and bacteria, - luminescent bacteria in symbiosis with fish and marine invertebrates)
Bibliography
The following list suggests some of the books that deal with the topics developed during the course (see program) all equally useful:
Dehò e Galli "Biologia dei microrganismi" Casa Editrice Ambrosiana (2018)
Brock. Biologia dei microrganismi. Microbiologia generale, ambientale e industriale. Ediz. mylab. Con espansione online (2016)
Willey et al “Prescott, Microbiologia” volumi 1-2-3 McGraw-Hill (2009)
As far as the Environmental Microbiology : Barbieri et al MICROBIOLOGIA AMBIENTALE ED ELEMENTI DI ECOLOGIA MICROBICA (2008) Casa Editrice Ambrosiana (Milano)
Theteacher will make available the slides shown and discussed during the lessons to facilitate the student in the task of synthesizing the study material.
The study on the textbook is however recommended either for attending and non attending students. The Library of the SCVSA Department is provided with copies available for loan.The teacher will publish on Elly platform the slides
Teaching methods
The course includes frontal lectures in class, explaining the general aspects of microbiology (from the structure of microbial cells to their metabolism, to the relationships between microorganisms and the environment). With this part of the course students will familiarize with the specific language of microbiology and with the most common methods used in laboratories.
Assessment methods and criteria
At the end of this course students will be able to
-understand basic principles of microbiology (cell structure and function, microbial genomics, microbial and metabolic diversity, microbial growth and growth control)
-describe, identify, and list the key distinguishing features of the major subgroups of both archaea and bacteria
-name, explain, and describe the core central metabolic and respiratory pathways in archaea and bacteria
-list major biogeochemical cycles, identifying those reactions that are microbe-catalyzed recognize the role of microorganisms in environmentally relevant processes including bioremediation of pollutants, biogeochemical cycling and wastewater treatment
-understand principles of microbial diversity and microbial ecological theory and how they apply to environmental microbiology
-know methods for studying microbial diversity and function and the different methods for the characterization of microbial communities
As described below, it will be verified that the learning objectives have been achieved and that the student is able to answer the open questions of the written test demonstrating that he is able to organize the acquired knowledge.
METHOD OF EXAMINATION Written test composed of 60 short questions (with a short answer).
Other information
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