At the end of the course, the student should have learned the basic techniques to manipulate in vitro the three main biological macromolecules: DNA, RNA and Proteins. The course also aims to teach the various strategies used in gene cloning in microorganisms and in the expression of recombinant proteins in bacteria.
In particular, the student should be able to:
D1. Understand and learn the methodologies for amplifying the three main classes of biological macromolecules: DNA, RNA and Proteins. Learn the composition and replication methods of different types of bacterial vectors. Understand the chemical and physical mechanisms of the recombinant technologies. (Knowledge and understanding)
D2. Design the most correct strategy to achieve gene cloning within a bacterial vector. Define the analytical methodologies to check for the gene cloning, and establish possible control experiments. (Applying knowledge and understanding)
D3. Know how to evaluate the best methodology to perform gene cloning or the expression of recombinant proteins. (Independent judgment)
D5. Expose the results of an experiment of recombinant technologies even to an unqualified public. (Communication skills)
D5. Know how to connect the molecular mechanisms learned during the courses of Biochemistry and Molecular Biology with the application methods of recombinant DNA. Update on innovative techniques by consulting scientific publications. (Learning ability)