The course aims to provide the methodology and the laboratory techniques employed by pathologists in the clinical practice and the research.
The main microscopic and molecular methods used in modern pathology will be discussed along with their diagnostic applications.
The acquired knowledge will allow the student to understand the variety of methodological approaches that can be applied to the study of pathological specimens. It will also allow the student to choose the more appropriate technique to the resolution of an experimental or diagnostic problem.
The student will be asked to integrate the notions provided by the course with previous knowledge in anatomy, molecular biology physiology and general pathology to the understanding of:
• the specificity and the relevance of a tissue approach to the study of disease;
• generalities on the molecular basis of cancer;:
• the physical, chemical and molecular premises to the use of the different morphological and molecular techniques;
• their main operative procedures;
• the logistic, instrumental and quality control requirements for their use in the diagnostic laboratory;
• their indications of use and main diagnostic applications.
At the end of the term, the student will be able to interact with other professional figures (clinicians, biotechnologists, pathologists, etc.) in the:
• choice of the more appropriate technique to solve a given experimental or diagnostic problem;
• critical interpretation of the results;
• integration of a tissue analysis approach in wider strategies of diagnosis and cure.