Learning objectives
The objective of this course is to provide students, through theoretical lectures, exercises in the classroom and work in laboratory, knowledge relating to principles of chemical equilibrium in aqueous solutions, volumetric analysis and main instrumental techniques.
Prerequisites
Knowledge of the basic principles of general and organic chemistry.
Course unit content
The course is composed of two sessions: 1th session "Analytical chemistry"; 2nd session "Instrumental analysis".
As for the session "Analytical chemistry", classes are articulated in theoretical lessons and classroom programming exercises. Main topics of the "Analytical chemistry " session are: preparation of standard solutions from pure solutes and by dilution of concentrated solutions; calculation of pH and concentration of the species present in aqueous solutions; principles of volumetric analysis.
As for the "Instrumental analysis" session, classes are focused on main instrumental methods as spectrophotometric and chromatographic methods. Great attention will paid on quality parameters of analytical methods (accuracy, precision, linearity, selectivity)
Full programme
"Analytical chemistry" session
-Ways of expressing the concentration of solutions: molarity, percentage by weight and by volume, parts per million and related. Preparation of standard solutions by weight and by dilution. Conversion between the various methods of expressing concentration levels. Exercises.
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Definition of acids and bases. Auto-ionisation and ionic product of water. Calculation of pH and concentration of the species present in the equilibrium of solutions of strong bases and acids. Exercises.
Definition of weak bases and acids. Dissociation constant. Calculation of pH and concentration of the species present in the equilibrium of solutions of weak bases and acids. Exercises.
Polyprotic acids. Calculation of pH and concentration of the species present in the equilibrium of solutions of polybasic acids. Distribution curve of equilibrium species on the basis of pH. Exercises.
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Calculation of pH of saline solutions. Exercises
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Buffer solutions. Calculation of pH of buffer solutions. Buffering capacity. Exercises.
- Principles of volumetric analysis: acid-base titration; oxidation-reduction titrations.
" Instrumental analysis"
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Spectrophotometric methods. Rotational, vibrational and electronic transitions. Absorption, emission, fluorescence. Lambert-Beer's law.
UV-Vis spectroscopy: principles, instrumentation and applications.
IR spectroscopy: principles, instrumentation and applications
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Chromatographic methods. General principles and theory of chromatographic separation. Fundamental parameters in chromatography.
Gas-chromatography (GC): principles, instrumentation and applications.
High pressure liquid chromatography (HPLC): adsorption chromatography, partition chromatography, size-exclusion chromatography, ion-exchange chromatography. Principles, instrumentation and applications.
Bibliography
Study materials (powerpoint presentations) are available on the Elly website. These presentations provide only a guideline of the topic discussed during classes but they should be integrated with the study of the suggested books available for library loan
Videolessons will be downloaded in Elly website
- P. Michelin Lausarot, G.A. Vaglio “Stechiometria per la chimica generale” Piccin
solo per la parte "analitica" Capitoli 7,13,14 e 16
- D.C. Harris “Chimica analitica quantitativa” Zanichelli
Per la parte di analitica paragrafi: 1.2;1.3;3.3;5.2;6.5;6.6;6.7;7.1;7.2;9 (tutto);11.1;11.2;11.3;11.6;11.8;12.1;12.2;12.7;16.4;16.5;16.6;16.7.
Per la parte di analisi strumentale paragrafi 18 (tutto); 20.1;20.2;20.3;23 (tutto);24
Teaching methods
Theoretical lectures and exercises will be performed in the classroom
Assessment methods and criteria
- "Analytical chemistry" session: written examination (solution of numerical exercices). Duration: two and half hours. Calculator will be allowed. Examples of exercices are available on the Elly website. Minimum score 18; maximum score 32 (30 cum laude). Exam will be prerformed in the classrom except in the case of sanitary emegency; in this case exam will be performed on line
- "Instrumental analysis" session: 32 multiple-choice questions quiz on the Elly website. Right answer= 1 point; wrong answer = -0.5 points; not given answer = 0 points. Duration 30 min. Calculator not allowed. Topics from "Analytical chemistry" session are also included in the quiz.
Quiz and written exams could be performed in the preferred order.
Final score is the average of both scores (written exercices + quiz)
Other information
Cause the COVID-19 emergency, teaching and examination possible changes will be promptly notified to the students
