Learning objectives
The student should (1) know the most commonly prescribed laboratory
analyses in clinical pathology, (2) understand their role in diagnostic
procedures, follow up and therapy monitoring, (3) properly evaluate their
cost/benefit ratio and (4) apply this information to relevant clinical
examples.
Prerequisites
Basic knowledge of biochemistry, molecular biology and virology is required
Course unit content
The course aims to provide specific knowledge in the field of laboratory techniques. In particular, topics of biochemistry and clinical molecular biology will be explored. This will allow understanding of the data production process, their interpretation, and their placement in the clinical setting.
Full programme
In particular, the following topics will be covered:
- Immunoenzymatic techniques: CLIA, ELISA, EIA
- Molecular biology techniques: PCR, RT-PCR, qPCR
- First and second level tests for the diagnosis and monitoring of HCV, HIV and HBV infections
- Impact of Biological samples choice on molecular investigations: a model in transplanted patients
- Application of new molecular technology in syndromic diagnosis: from the specimen to the correct diagnosis – example in clinical cases
- Mass spectrometry (MALDI-TOFF): method and its applications in clinical laboratory
Bibliography
All the links for further information will be provided during the frontal lessons
Teaching methods
The course will be conducted through interactive lectures in front of the
students, with the aid of illustrative material that, at the end of the
course, will be made available to students on Elly site at the pages of the
course.
Lectures will be held on-site in compliance with safety standards,
provided that further instructions are not implemented.
During the lessons we will discuss the issues related to laboratory tests
and their interpretation topic will be exposed, encouraging students to
ask questions on the basis of references that will be provided.
Assessment methods and criteria
Verification tests in progress will not be carried out.
The assessment of the achievement of the objectives of the course will
include a test with multiple choice quiz on topics listed in the extended
program.
Other information
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