Learning objectives
Gain a detailed understanding on:
functional anatomy of the components that structure the stem cell niches in different organs in the adult;
of structural and functional components of the niches of stem cells and progenitors for regeneration and repair of tissues differentiated;
techniques of isolation, characterization, expansion and differentiation of progenitor osteogenic, chondrogenic, hematopoietic, endocrine organs, skin and eye;
structural, molecular and cellular biomechanis of biomaterials in regenerative medicine
Knowledge and understanding.
Students need learn the fundaments of the structure and component of the stem cell niches in different organs in the adult, protocols and techniques for the regeneration of differentiated tissues and in particular the eye and the oral cavity components, the bone tissue, the skin, and the endocrine organs.
Applying knowledge and understanding
Students must use molecular and cellular techniques for development of a protocol for the isolation, characterization, expansion and differentiation of stem cells and primary culture for the tissue regeneration.
Making judgments
Ability to reason about molecular and cellular protocol for cell niche and stem cell study appeared on an article of an international journal and/or ph-thesis
Communications skills
Ability to understand and be understood in technical situations
Learning skills
Ability to summarize a technical procedure and to write a scientific progress report
Prerequisites
Humana anatomy and histology
Course unit content
First lessons: Cell isolation and culture. Functional anatomy of the stem cell niche, characterization of stem cell and stem cell niche of the liver, kidney, nervous system, lung, thymus and skeletal muscle.
Subsequent lessons: the regenerative medicine in endocrinology, the biomaterials and cell culture for the regeneration of endocrine and neuroendocrine tissues, the skin regeneration techniques and the immunobiology of the skin.
Latest lessons: techniques of oral tissue regeneration, techniques in corneal transplantation, techniques of bone regeneration
Full programme
Functional anatomy of the stem cell niche.
The corneal and retinal stem cells.
The epidermal stem cell
The hepatic stem cell niche,
The renal stem cell niche,
The neuronal stem cell niche,
The bronchioalveolar stem cell niche
The thymic stem cell niche,
The skeletal muscle stem cell niche,
Functional anatomy of the hemolymphopoietic organs
Regenerative medicine in endocrinology: biomaterials and the regeneration of endocrine and neuroendocrine tissues.
Ex situ bioengineering of endocrine and neuroendocrine organs.
The skin regeneration techniques: immunobiology and skin regeneration.
Techniques of oral tissue regeneration
Biomaterials and implant prosthetics and tissue regeneration in periodontology
Techniques of bone regeneration
The biomechanic, structure, molecular and cellular properties of the bone.
The osteogenic molecules in the regeneration and / or the ossteointegration between bones and stromal cells and biomaterials.
Regeneration techniques in eye diseases
New techniques in corneal transplantation.
Bibliography
International reviews and scientific commentaries will dispensed by the teacher
issues.
Teaching methods
Oral lesson, lectures. Assimilation of scientific literature concerning the methods, the experimental protocols, the animal models, stem cell culture isolation and characterization procedures.
Assessment methods and criteria
End-of-term evaluation and continuous assessment. The assessment of the achievement of the objectives of the course consists of an written examination. Through questions about the contents of the course will be determined whether the student has achieved the goal of knowledge and understanding of the content.
Written examination by a multiple (four) choice tests. 32 questions on the stem cell, the niche anatomy, the tissue regeneration and tissue engineering. Students must answer correctly at least to 18 questions.
Other information
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