Learning objectives
The students will become familiar with different types of instruments and
techniques needed for the microscopic analysis and cultivation of bacteria, fungi,
parasites and viruses
Prerequisites
- - -
Course unit content
The student has to know the laboratory skills to study the morphological and growth
properties of bacteria, fungi, parasites and viruses of medical relevance.
The student has to observe and interpret slides containing bacteria, fungi and
parasites, as well as photographic sheets containing viruses. Moreover, the student
has to observe examples of cultures of bacteria, fungi, parasites and viruses.
Obiettivi formativi The students will become familiar with different types of instruments and
techniques needed for the microscopic analysis and cultivation of bacteria, fungi,
parasites and viruses
Microbiology laboratory training
Professionalizing training: Legend (L): 0: The student has seen the execution, 1: The student has executed at least one time, 2: The student is able to execute autonomously.
CLINICAL MICROBIOLOGY
Prescription of microbiological and virological investigations 2
Interpretation of the report of the microscopic examination for bacteria (Gram staining) 2
Interpretation of the report of the microscopic examination for bacteria (Ziehl-Nielsen staining) 2
Interpretation of the report of the culture for bacteria 2
Interpretation of the report of the antibiogram for bacteria 2
Interpretation of the report for the serological diagnosis of syphilis 2
Interpretation of the report for the diagnosis of tubercular infection 2
Interpretation of the report for the detection of nucleic acids of bacteria 2
Interpretation of the report of the culture for fungi 2
Interpretation of the report of the antibiogram for fungi 2
Interpretation of the report for the detection anti-Toxoplasma gondii antibodies 2
Interpretation of the report for the detection of intestinal parasites 2
Interpretation of the report for the detection of intestinal parasites 2
Interpretation of the report for the detection of Leishmania 2
Execution of the thin smear of peripheral blood 0
Interpretation of the report for the detection of nucleic acids of protozoa 2
Electrophoresis in agarose gel of nucleic acids of protozoa 0
Interpretation of the report for the detection of antigens of protozoa 2
Interpretation of the report for the diagnosis of malaria 2
Observation of arthropod parasites by macroscopic examination 1
Observation of elmynth parasites (Cestodes, Nematodes) by macroscopic examination 1
Interpretation of the report for the diagnosis of viral hepatitis 2
Interpretation of the report for the detection of antigens of hepatitis viruses 2
Interpretation of the report for the detection of nucleic acids of hepatitis viruses 2
Interpretation of the report for the detection of anti-hepatitis viruses antibodies 2
Electrophoresis in agarose gel of nucleic acids of viruses 0
Interpretation of the report for the detection of viruses by electron microscopy 2
Interpretation of the report for the detection of anti-viruses antibodies 2
Interpretation of the report of the culture for viruses 2
Interpretation of the report for the detection of antigens of viruses 2
Interpretation of the report for the detection of antigens of HIV 2
Interpretation of the report for the detection of nucleic acids of HIV 2
Interpretation of the report for the detection of anti-HIV antibodies 2
To begin to apply the logical tools in the clinical reasoning and to address the choices of the diagnostic tools according to the decisional criterions of efficiency and effectiveness that keep in mind of the relationships among benefits, risks and costs.
- Preanalytical, analytical and postanalytical variability. Condition that can alter the results of the analyses of laboratory; different possibilities of collecting.
- Correct application of the techniques for the collecting and the harvest of the materials.
- The concept of normality in biology and the functions of risk (normal values on statistic base, normal values on biological base, desirable values and functions of risk
Full programme
- - -
Bibliography
M. Bendinelli, C. Chezzi, G. Dettori, N. Manca, G. Morace, L. Polonelli, M.A. Tufano - "MICROBIOLOGIA MEDICA: BATTERIOLOGIA, MICOLOGIA, VIROLOGIA. Gli agenti infettivi dell'uomo: biologia, azione patogena, laboratorio. " Monduzzi Editore -
Teaching methods
Microbiology laboratory training
Professionalizing training: Legend (L): 0: The student has seen the execution, 1: The student has executed at least one time, 2: The student is able to execute autonomously.
CLINICAL MICROBIOLOGY
Prescription of microbiological and virological investigations 2
Interpretation of the report of the microscopic examination for bacteria (Gram staining) 2
Interpretation of the report of the microscopic examination for bacteria (Ziehl-Nielsen staining) 2
Interpretation of the report of the culture for bacteria 2
Interpretation of the report of the antibiogram for bacteria 2
Interpretation of the report for the serological diagnosis of syphilis 2
Interpretation of the report for the diagnosis of tubercular infection 2
Interpretation of the report for the detection of nucleic acids of bacteria 2
Interpretation of the report of the culture for fungi 2
Interpretation of the report of the antibiogram for fungi 2
Interpretation of the report for the detection anti-Toxoplasma gondii antibodies 2
Interpretation of the report for the detection of intestinal parasites 2
Interpretation of the report for the detection of intestinal parasites 2
Interpretation of the report for the detection of Leishmania 2
Execution of the thin smear of peripheral blood 0
Interpretation of the report for the detection of nucleic acids of protozoa 2
Electrophoresis in agarose gel of nucleic acids of protozoa 0
Interpretation of the report for the detection of antigens of protozoa 2
Interpretation of the report for the diagnosis of malaria 2
Observation of arthropod parasites by macroscopic examination 1
Observation of elmynth parasites (Cestodes, Nematodes) by macroscopic examination 1
Interpretation of the report for the diagnosis of viral hepatitis 2
Interpretation of the report for the detection of antigens of hepatitis viruses 2
Interpretation of the report for the detection of nucleic acids of hepatitis viruses 2
Interpretation of the report for the detection of anti-hepatitis viruses antibodies 2
Electrophoresis in agarose gel of nucleic acids of viruses 0
Interpretation of the report for the detection of viruses by electron microscopy 2
Interpretation of the report for the detection of anti-viruses antibodies 2
Interpretation of the report of the culture for viruses 2
Interpretation of the report for the detection of antigens of viruses 2
Interpretation of the report for the detection of antigens of HIV 2
Interpretation of the report for the detection of nucleic acids of HIV 2
Interpretation of the report for the detection of anti-HIV antibodies 2
To begin to apply the logical tools in the clinical reasoning and to address the choices of the diagnostic tools according to the decisional criterions of efficiency and effectiveness that keep in mind of the relationships among benefits, risks and costs.
- Preanalytical, analytical and postanalytical variability. Condition that can alter the results of the analyses of laboratory; different possibilities of collecting.
- Correct application of the techniques for the collecting and the harvest of the materials.
- The concept of normality in biology and the functions of risk (normal values on statistic base, normal values on biological base, desirable values and functions of risk, usable decisional values to diagnostic or therapeutic end).
- General principles of the most diffused techniques in use near the clinical laboratories. Control of quality.
- To appraise critically a report (intervals of reference, sensibility and specificity of a test, predictive value, critical difference). The values of reference of the analyses of the common routine.
- Elements of diagnostic molecular.
- Investigations for the control of the pregnancy.
- Interpretation of the complete examination of the urines in key of diagnostic use.
- Interpretation of measures of enzymes
Assessment methods and criteria
- - -
Other information
- - -
