BIOCHEMICAL-CLINICAL ANALYSIS
cod. 18374

Academic year 2020/21
2° year of course - Second semester
Professor
- Franco BACCIOTTINI
Academic discipline
Biochimica clinica e biologia molecolare clinica (BIO/12)
Field
Attività formative affini o integrative
Type of training activity
Related/supplementary
40 hours
of face-to-face activities
5 credits
hub: PARMA
course unit
in ITALIAN

Learning objectives

This course aims to provide the informations and the scientific underground to approach correctly the main topics in Clinical Biochemistry by students of pharmaceutical sciences.
This knowledge is needed especially by a professional like the chemist which is at the connection between patients and medical structures; in this context , appropriate communicative skills are needed to explain the results of biochemical lab analysis and to orientate patients between many specific diagnostic devices and tools for specimen’s collection, treatment and managing commonly available in the chemist shop.

Prerequisites

There are no obligatory prerequisites, but Clinical Biochemistry is an interdisciplinary science and needs the basic knowledge in Biology, Anatomy, Physiology, Chemistry and Biochemistry

Course unit content

Pre-analytical, analytical and post-analytical variability. Types of biological samples and optimal procedures to collect them. Variables affecting laboratory data
The concept of normal values in biology and the risk functions (normal values stated by statistical calculations or by biological observations; desirable values and risk function; decisional values for diagnostic or therapeutic purpose).
Interpretation of the complete urinalysis test.
Interpretation of enzymatic and iso-enzymatic tests like auxiliary diagnostic tool in some specific organ disease.
Laboratory test modifications by diseases affecting purine metabolism.
Laboratory test modifications by diseases affecting the hydro-electrolyte balance.
Interpreting the variations of acid-base parameters and possible pathological conditions.
Interpretation of the modifications of plasma protein profile.

Full programme

Part 1. General Clinical Chemistry.
Biological specimens consideration: instruction for optimal collection, treatment and conservation (avoiding pre-analytical variation).
Part 2. Analysis of biological samples; analytical techniques and instrumentations.
The main analytical techniques and instrumentations to evaluate the molecules of interest in Clinical Chemistry. Analytical and post-analytical variability affecting the laboratory data. Clinical laboratory quality assurance. Kits for diagnostic tests.
Part 3. Current waived test.
Point of care testing (POCT): use and application. Criteria for test waiving. Quality control in POCT. Implementation and monitoring of POCT. Specimen and its treatment in POCT. Places of POCT utilization. Auto-monitoring of some biochemical parameters.
Part 4. Application of POCT
Control of glycemia; Diabetes and patient auto-monitoring. Lipid metabolism diseases; POCT for blood triglycerides, cholesterol and lipoprotein analysis; auto-monitoring and cardiovascular risk.
Part 5. Outline of Clinical Biochemistry
Markers of function or damage of some organs and systems. Index of cytolysis, clinical enzymology. Main cardiac, hepatic and renal markers

Bibliography

- Albi E. Biochimica Clinica Essenziale. Zanichelli
- Kellerman G. Valori anormali di laboratorio. Mc Graw Hill
- Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Elsevier Saunders, St.Louis (Missouri)-USA.

Teaching methods

Interactive lessons.
Persisting the sanitary emergency we opt also for synchronous online lessons via Teams.
Weekly lessons are devoted to explanations if requested

Assessment methods and criteria

The final oral examination is about the topics of the course; the student needs to demonstrate to have understood and to be able to manage the essential meanings of every section of the program.
Persisting the sanitary emergency we opt also for online oral test via Teams: one question about general topics and one on more specialistic subjects.

Other information

The iconographic supports shown during the lessons will be available (on Elly) to the students since the beginning of the course