LABORATORY MEDICINE TECHNICAL SCIENCES
cod. 1002594

Academic year 2022/23
5° year of course - First semester
Professor
- Adriana CALDERARO
Academic discipline
Scienze tecniche di medicina di laboratorio (MED/46)
Field
Formazione clinica interdisciplinare e medicina basata sulle evidenze
Type of training activity
Characterising
10 hours
of face-to-face activities
1 credits
hub: PARMA
course unit
in ITALIAN

Integrated course unit module: LABORATORY MEDICINE

Learning objectives

CLINICAL MICROBIOLOGY
Principles and methods for the laboratory diagnosis of infectious diseases
Aetiology, outline of pathogenesis, clinical samples, laboratory diagnostic
procedures, interpretation of the results of microbiological and virological
investigations to diagnose the infectious diseases.
The determination of susceptibility/resistance of the pathogenic agents to
chemotherapeutics

Prerequisites

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Course unit content

Principles and methods for the laboratory diagnosis of infectious diseases
- Demonstration of the pathogenic agent and its constituents in the pathological material
- Demonstration of a specific immune response
- Collection, transport and preservation of the clinical samples for microbiological and virological
investigations
Description, demonstration and interpretation of the results of microbiological and virological investigations. In particular, the following methods will be described:
DIRECT INVESTIGATION (demonstration of the agent or its components in the biological sample).
- Immunocromatographic assays, immunoenzimatic assays and immunofluorescence assays for the research of specific bacterial, viral fungal and parasitic antigens;
- In situ hybridization assays for the research of the ribosomal RNA of bacteria and yeasts from culture;
- Microscopic examination (fresh or after coloration) for bacteria, fungi and parasites; transmission electronic microscopy for the research and identification of viruses;
- Culture for bacteria, fungi and parasites; cell culture of viruses;
- Identification by MALDI-TOF mass spectrometry of bacteria and fungi;
- Research for bacterial, fungal and parasitic DNA directly and from culture, research for the nucleic acid of viruses for identification and/or quantification purposes in the biological sample;
- Research for the mycobacterial DNA and for the nucleic acid of viruses for the determination of the genotype and the drug resistance;
- The determination of susceptibility/resistance of the pathogenic agents to chemotherapeutics
INDIRECT INVESTIGATION.
- Determination of an anti-bacterial, anti-fungal, anti-parasitic anti-viral specific immune response by immunoenzimatic assays, agglutination assays and Western blot assays.

Full programme

CLINICAL MICROBIOLOGY
Principles and methods for the laboratory diagnosis of infectious diseases -
Demonstration of the pathogenic agent and its constituents in the pathological
material - Demonstration of a recent specific immune response - The medical
prescription of microbiological and virological investigations - Collection, transport
and preservation of the clinical samples for microbiological and virological
investigations - Aetiology, outline of pathogenesis, clinical samples, laboratory
diagnostic procedures, interpretation of the results of microbiological investigations
regarding the diagnosis of:
Infections of the respiratory apparatus - Infections of the intestinal apparatus and
related glands - Systemic infections - Infections of the cardiovascular apparatus -
Infections of the central nervous system - Infections of the genitourinary apparatur
and sexually - transmitted infections - Infections of the skin and cutaneous adnexa -
Infections of the muscular-skeletal apparatus - Infections of the eye and the earprenatal,
connatal and perinatal infections - The nosocomial infections - The
determination of susceptibility/resistance of the pathogenic agents to
chemotherapeutics - Methods - Interpretation of the results - The dosage of
chemotherapeutics in tissues and biological fluids.

Bibliography

M.Bendinelli, A. Calderaro, C. Chezzi, G. Dettori, N. Manca, G. Morace, L. Polonelli, M.A. Tufano - - Monduzzi Editore - 2006-2012 - Microbiologia Medica: Batteriologia, Micologia, Parassitologia, Virologia. Gli agenti infettivi dell'uomo: biologia, azione patogena, laboratorio.

Teaching methods

Oral and practical lesson in clinical diagnostic laboratoriesLectures will be held on-site in compliance with safety standards, provided that further instructions on the ongoing health emergency are not implemented. Supporting material will be available on the specific, student-reserved platform (Elly) and will include slide presentations, audio-video aids or video-recording of the lectures.

Assessment methods and criteria

Oral examination

Other information

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