Learning objectives
CLINICAL MICROBIOLOGY
Principles and methods for the laboratory diagnosis of infectious diseases
Aetiology, outline of pathogenesis, clinical samples, laboratory diagnostic
procedures, interpretation of the results of microbiological and virological
investigations to diagnose the infectious diseases.
The determination of susceptibility/resistance of the pathogenic agents to
chemotherapeutics
Prerequisites
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Course unit content
Principles and methods for the laboratory diagnosis of infectious diseases
- Demonstration of the pathogenic agent and its constituents in the pathological material
- Demonstration of a specific immune response
- Collection, transport and preservation of the clinical samples for microbiological and virological
investigations
Description, demonstration and interpretation of the results of microbiological and virological investigations. In particular, the following methods will be described:
DIRECT INVESTIGATION (demonstration of the agent or its components in the biological sample).
- Immunocromatographic assays, immunoenzimatic assays and immunofluorescence assays for the research of specific bacterial, viral fungal and parasitic antigens;
- In situ hybridization assays for the research of the ribosomal RNA of bacteria and yeasts from culture;
- Microscopic examination (fresh or after coloration) for bacteria, fungi and parasites; transmission electronic microscopy for the research and identification of viruses;
- Culture for bacteria, fungi and parasites; cell culture of viruses;
- Identification by MALDI-TOF mass spectrometry of bacteria and fungi;
- Research for bacterial, fungal and parasitic DNA directly and from culture, research for the nucleic acid of viruses for identification and/or quantification purposes in the biological sample;
- Research for the mycobacterial DNA and for the nucleic acid of viruses for the determination of the genotype and the drug resistance;
- The determination of susceptibility/resistance of the pathogenic agents to chemotherapeutics
INDIRECT INVESTIGATION.
- Determination of an anti-bacterial, anti-fungal, anti-parasitic anti-viral specific immune response by immunoenzimatic assays, agglutination assays and Western blot assays.
Full programme
CLINICAL MICROBIOLOGY
Principles and methods for the laboratory diagnosis of infectious diseases -
Demonstration of the pathogenic agent and its constituents in the pathological
material - Demonstration of a recent specific immune response - The medical
prescription of microbiological and virological investigations - Collection, transport
and preservation of the clinical samples for microbiological and virological
investigations - Aetiology, outline of pathogenesis, clinical samples, laboratory
diagnostic procedures, interpretation of the results of microbiological investigations
regarding the diagnosis of:
Infections of the respiratory apparatus - Infections of the intestinal apparatus and
related glands - Systemic infections - Infections of the cardiovascular apparatus -
Infections of the central nervous system - Infections of the genitourinary apparatur
and sexually - transmitted infections - Infections of the skin and cutaneous adnexa -
Infections of the muscular-skeletal apparatus - Infections of the eye and the earprenatal,
connatal and perinatal infections - The nosocomial infections - The
determination of susceptibility/resistance of the pathogenic agents to
chemotherapeutics - Methods - Interpretation of the results - The dosage of
chemotherapeutics in tissues and biological fluids.
Bibliography
M.Bendinelli, A. Calderaro, C. Chezzi, G. Dettori, N. Manca, G. Morace, L. Polonelli, M.A. Tufano - - Monduzzi Editore - 2006-2012 - Microbiologia Medica: Batteriologia, Micologia, Parassitologia, Virologia. Gli agenti infettivi dell'uomo: biologia, azione patogena, laboratorio.
Teaching methods
Live and streaming oral and practical lesson in clinical diagnostic laboratories.
Assessment methods and criteria
Oral examination
Other information
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2030 agenda goals for sustainable development
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