Course catalogue | University of Parma

Professor
Academic discipline: Biochimica (BIO/10)
Field: Discipline biologiche e farmacologiche
Type of training activity: Characterising

64 hours
of face-to-face activities

8 credits

hub: PARMA

course unit
in - - -

lectures timetable unavailable

exam calls

Learning objectives

The course aims at highlighting specific topics of advanced biochemisty that might be of interest to students of the Faculty of Pharmacy

Prerequisites

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Course unit content

Part I: Biochemical techniques
Protein purification: centrifugation and chromatography. Electrophoresis. 2D electrophoresis and mass spectrometry.
Spectroscopy in biochemistry. Absorbtion spectroscopy, fluorescence, circular dichroism.
Enzyme assays. The steady state. Direct, indirect and coupled enzyme assays. Pre-steady state kinetics.
Immunochemical techniques. The antibodies (structure, conjugation). Production of monoclonal and polyclonal antibodies. Immunoassays: RIA and ELISA.
Recombinant DNA. Recombinant proteins. Recombinant proteins as drugs.
Protein structure determination. Structure and sequence databanks.
Computational methods for drug design.
Part II: Cellular
biochemistry
Membrane receptor
Singnal trsnduction pathways
Lipid hormons
Nuclear receptors
Misfolding diseseas
Blood substitutes
Examples of scientific projects on proteomics, enzyme catalysis, drug design.

Full programme

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Bibliography

Material provided by the teacher

Berg, Tymoczko: “Stryer – Biochimica”, Zanichelli

Wilson e Walker: “Biochimica e Biologia Molecolare", Nuova edizione italiana. Cortina Editore.

G. Federici: “Medicina di laboratorio”, Edizione McGraw- Hill

Teaching methods

Frontal lectures

Assessment methods and criteria

Oral examination and scientific paper presentation

Other information

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APPLIED BIOCHEMISTRY cod. 00062

Learning objectives

Prerequisites

Course unit content

Full programme

Bibliography

Teaching methods

Assessment methods and criteria

Other information

APPLIED BIOCHEMISTRY
cod. 00062