Learning objectives
Aims of the course is the study of genetic approaches applied to genomics and functional analysis in microorganisms. Particular attentions will be paied to model organisms in fundamental and biotechnologic applications.
Prerequisites
None
Course unit content
YEAST AS MODEL ORGANISM
Forward e reverse genetics- Genome sequencing in S. cerevisiae- Comparative genomics in yeasts- S. cerevisiae deletant collection and use of null mutants in functional analysis- Methods for gene interaction studies applied to human diseases - Yest as model organism for the study of mitochondrial genetics- Yeast as model organism for the study of mitochondrial diseases.
FUNCTIONAL ANALYSIS IN PLANTS
Caenorhabditis elegans AS MODEL ORGANISM
Forward e reverse genetics in C. elegans. Functional analysis by RNAi - C. elegans as model organism for the study of human pathologies.
Drosophila melanogaster AS MODEL ORGANISM
Forward e reverse genetics in D. melanogaster. - D. melanogaster as model organism for the study of human pathologies.
HUMAN GENETICS. HUMAN GENETIC DISEASES.
PHARMACOGENETICS. GENE THERAPY.
Full programme
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Bibliography
P. Meneely
Analisi Genetica Avanzata
Ed. McGraw-Hill
Original papers selected by the teacher
E.H.McCONKEY – Genetica Umana. Una prospettiva molecolare – Zanichelli
Teaching methods
frontal lectures and exercises
Assessment methods and criteria
Tests during the course and final oral examination
Other information
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