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Università degli Studi di Parma Università degli Studi di Parma
University of Parma
Course catalogue
Università degli Studi di Parma
course catalogue

GENOMICS AND TRANSCRIPTOME ANALYSIS METHODS
cod. 1001184

Academic year 2011/12
2° year of course - First semester
Professor responsible for the course unit
MARMIROLI Nelson
integrated course unit
9 credits
hub: PARMA
course unit
in - - -
lectures timetable unavailable
exam calls

Course unit structured in the following modules:

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Learning objectives

To provide knowledge on the methodologies of functional genomics and their possible applications

Prerequisites

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Course unit content

What is a genome
Sequencing genomes
How to interpret information in a genomic sequence (in silico methods)
Genome anatomy
Transcription of complex genomes (human, animal, plant)
Synthesis and processing of RNA
Regulation of genome activity
Analysis of transcriptome
Molecular arrays and methods for analysis of arrays
Other methods for analysing the transcriptome (differential display, cDNA-AFLP, SAGE)
Quantification of the transcriptome
Methodologies in RealTime and theoretical bases
Genetic-molecular methods to study the function of individual genes (two hybrid, phage display, genetic footprinting, gene inactivation)
Site-specific mutagenesis
In vivo methods to analyse gene function in higher organisms (plant and animals)
Molecular methodologies to obtain knock-in, knock-out animals and mutagenised plants
Applications of functional genomics to pharmacology, medicine, agriculture, environment

Full programme

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Bibliography

material provided by the lecturer

Teaching methods

frontal lectures

Assessment methods and criteria

oral examination

Other information

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