Learning objectives
The purpose is to have a complete information about different procedures used in diagnostic microbiology: direct diagnosis, isolation and characterization, serological diagnosis, several possibilities of molecular diagnosis using different probe assaies, PCR and actual modifications.
Prerequisites
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Course unit content
<br />Systematic taxonomy of microrganisms causing infecion in human host.<br />Different tropism of pathogens related to infection in humans.<br />Microbiological diagnosis.<br />Procedures for a correct microbial diagnosis.<br />Growth and isolation of pathogens: its characterisation.<br />Determination of specific antibodies production,seroconversion, and description of different related methodologies.<br />Procedures using specific molecular probes.<br />Polymerase chain reaction and its actual modification to gain better results. <br />Quantitative PCR.<br />Antimicrobial compounds and its “in vivo” determination in body fluids.<br />Determination of “in vitro” sensitivity and resistance to antimicrobials.<br />Vaccinetherapy and Serotherapy.<br />Antiviral compounds.
Full programme
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Bibliography
<br />Mandell G. et al., Principles and practice of infectious diseases, 2000, Churchill Livingstone. <br />Roitt et al., Immunology, 5th Ed., 1997
Teaching methods
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Assessment methods and criteria
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Other information
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