Medical, veterinary anf pharmaceutical biotechnologies

Professor
Academic discipline: Patologia generale (MED/04)
Field: Discipline biotecnologiche comuni
Type of training activity: Characterising

24 hours
of face-to-face activities

3 credits

hub: -

course unit
in - - -

lectures timetable unavailable

exam calls

Integrated course unit module: IN VIVO AND IN VITRO TESTING MODELS

Learning objectives

This core module aims to introduce students to the concepts of ex vivo ed in vitro
cell models used to study pathological processes involved in the major diseases.

Prerequisites

- - -

Course unit content

-Organ and tissue culture
-Extracellular matrix and tissue dissociation
-Cell purification
-Cell lines
-Cell cloning
-Culture of animal cells
-Culture contamination
-Gene transfer
-Cell vitality and cell proliferation assays
-Citotoxicity assays
-Organotypic culture models

Full programme

Strategy of cell cultures
Biology of cultured cells.
Advantages and disadvantages of cell culture.
The environment of the cell culture: substrates, media, temperature, growth factors,
etc.. "Scaling-up of cell cultures.
Kinetics of growth. Survival and toxicity.
Tissue dissociation and primary cultures.
Separation and characterization of specific cell types.
Maintenance of cultures.
Cryopreservation
Contamination of cell cultures.
The cancer cell: morphological and metabolic features.
Established cell lines.
Methods for transferring therapeutic genes
• transfection by physical methods: microinjection, electroporation, bombardment
(gene gun)
• Lipofezione: liposomes, cationic lipid-DNA complexes
• viral vectors: retrovirus, adenovirus, adeno-associated virus

Bibliography

R. Ian Freshney, Culture of Animal Cells: A Manual of Basic Technique, 5th Edition,
ISBN: 0-471-45329-3 Ed Wiley-
Jan-Thorsten Schantz & Kee Woei Ng: A MANUAL FOR PRIMARY HUMAN CELL
CULTURE - ISBN 981-238-796-X(pbk) ed. World Scientific
Alan Doyle , J. Bryan Griffiths: Cell and Tissue Culture: Laboratory Procedures in
Biotechnology ISBN: 0-471-98255-5- Ed Wiley

Teaching methods

Interactive oral lesson. Iconographic support will be provided.

Assessment methods and criteria

Oral examination

Other information