Learning objectives
The course aims to give at the student a specific knowledge of biotechnological techniques applied to morphological and functional study of tissues, in particular bone tissue.
Prerequisites
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Course unit content
Theoretical part: <br />
Locomotor system: classification of bones and principles of biomechanic <br />
Osteology: <br />
- Forms of bony tissue <br />
- Histogenesis <br />
- Morphological and structural characteristics <br />
- Deposition and remodeling dinamics <br />
Definition and classification of biomaterials employed in orthopaedic and dental field <br />
Experimental protocols for evaluation of osteointegration <br />
Notions of legislation on animal experimentation <br />
Methods for the osteintegration evaluation: bone vital markers, polarized light microscopy analysis, histochemical and immunohistochemical reactions, histological stains. <br />
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Practical part: <br />
The practical activity will focused on: <br />
- Histological techniques for processing undecalcified bone tissue with implants <br />
- Histolgical stains, Histochemical and Immunohistochemical reactions <br />
- Observation of histological sections by polarizing light and fluorescence optical microscope <br />
- Digitalization and image analysis. <br />
Full programme
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Bibliography
The didactical material, consisting of scientific publications and experimental protocol, will be provided during the course.
Teaching methods
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Assessment methods and criteria
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Other information
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