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University of Parma
Course catalogue
Università degli Studi di Parma
course catalogue

APPLIED BIOCHEMISTRY
cod. 00062

Academic year 2011/12
3° year of course - First semester
Professor
Academic discipline
Biochimica (BIO/10)
Field
Discipline biologiche e farmacologiche
Type of training activity
Characterising
64 hours
of face-to-face activities
8 credits
hub: PARMA
course unit
in - - -
lectures timetable unavailable
exam calls
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Learning objectives

The course aims at highlighting specific topics of advanced biochemisty that might be of interest to students of the Faculty of Pharmacy

Prerequisites

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Course unit content

Part I: Biochemical techniques
Recombinant DNA. Recombinant proteins. Recombinant proteins as drugs.
Protein purification: centrifugation and chromatography. Electrophoresis. 2D electrophoresis and mass spectrometry.
Spectroscopy in biochemistry. Absorbtion spectroscopy, fluorescence, circular dichroism.
Enzyme assays. The steady state. Direct, indirect and coupled enzyme assays. Pre-steady state kinetics.
Immunochemical techniques. The antibodies (structure, conjugation). Production of monoclonal and polyclonal antibodies. Immunoassays: RIA and ELISA.
Protein structure determination. Structure and sequence databanks. An introduction to bioinformatics.

Part II: Clinical biochemistry
Clinical biochemistry: a definition.
Biological samples. Analytes. Units of measurements. Reference intervals.
Clinical biochemistry in the assessment of organ function and disease (heart, kidney, liver, blood).
Tumor markers.

Part III: The biochemistry of drug targets
Drugs and drug targets: an introduction.
DNA as a drug target.
RNA as a drug target. Antisense nucleotides and RNA interference. Antibiotics binding ribosomal RNA.
The membrane lipid bilayer as a drug target.
Proteins as drug targets: enzymes. Receptors and signaling pathways. Transmembrane carriers, pumps and channels.

Full programme

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Bibliography

Material provided by the teacher

Berg, Tymoczko: “Stryer – Biochimica”, Zanichelli

Wilson e Walker: “Biochimica e Biologia Molecolare", Nuova edizione italiana. Cortina Editore.

G. Federici: “Medicina di laboratorio”, Edizione McGraw- Hill

Teaching methods

Frontal lectures

Assessment methods and criteria

Oral examination

Other information

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