Vai al contenuto principale Vai al footer
Università degli Studi di Parma Università degli Studi di Parma
University of Parma
Course catalogue
Università degli Studi di Parma
course catalogue

MOLECULAR BIOLOGY
cod. 02617

Academic year 2008/09
1° year of course - First semester
Professor
Academic discipline
Biologia molecolare (BIO/11)
Field
Ambito aggregato per crediti di sede
Type of training activity
Hub-specific activity
15 hours
of face-to-face activities
1 credits
hub: -
course unit
in - - -
lectures timetable unavailable
exam calls

Integrated course unit module: MOLECULAR BIOLOGY

Print

Learning objectives

Students, starting from the knowledge of DNA structure and chemical composition, will learn the advanced techniques of modern molecular biology applied in biotechnology and research.

Prerequisites

no

Course unit content

<strong>The bacterial cell</strong>: approach to the technology of recombinant DNA. <br />
<strong>DNA, the primary genetic material</strong> <br />
The structure of DNA, genetic code and protein synthesis. Role of genes in cells. Notions of gene expression and regulation. <br />
<strong>Techniques of DNA analysis and manipulation</strong> <br />
Restriction enzymes, electrophoretic separation. Modification enzymes, in vitro reactions. <br />
<strong>Recombinant DNA and cloning strategies</strong> <br />
Cloning vectors; bacterial competence and transformation. <br />
<strong>Hybridization techniques <br />
</strong>Probes and hybridization: a cDNA library. Northern e Southern Blotting techniques. <br />
<strong>The Polymerase Chain Reaction</strong> <br />
PCR technology and applications. DNA sequencing. <br />
<strong>Applications of recombinant DNA technology</strong> <br />
Procariotic expression vectors: pUC and pET plasmids. Few concepts of recombinant expression and protein engineering. <br />

Full programme

- - -

Bibliography

<p>Allison L.A. Fondamenti di Biologia Molecolare, Zanichelli Ed.</p>
<p>All the study materials and supporting web sites are provided by the teacher.</p>

Teaching methods

Frontal lessons aided by presentations and animations files. Oral exam.

Assessment methods and criteria

- - -

Other information

- - -