Learning objectives
In-depth insights of qualifying aspects instrumental to the acquisition of more specific expertise for the professional approach to virological diagnosis.
Prerequisites
No
Course unit content
In vitro cultivation of viruses: diploid and continuous cellular lines, preparation of cellular substrates. Virus-induced cytophatic effect: definition, causes, examples, identification. Immunofluorescence reaction: direct, indirect. Research and identification of viral proteins. Transmission electron microscopy: general principles, preparation of grids, negative staining, virus observation.
Full programme
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Bibliography
M. Bendinelli, C. Chezzi,, G. Dettori, N. Manca, G. Morace, L. Polonelli, M.A. Tufano. Microbiologia Medica. Monduzzi Editore. <br />
<br />
Wild P. <br />
Electron microscopy of viruses and virus-cell interactions. <br />
Methods Cell Biol. 2008;88:497-524. <br />
<br />
Hazelton PR, Gelderblom HR. <br />
Electron microscopy for rapid diagnosis of infectious agents in emergent <br />
situations. <br />
Emerg Infect Dis. 2003 Mar;9(3):294-303. <br />
Teaching methods
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Assessment methods and criteria
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Other information
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