CELLULAR MODELS AND GMOS
cod. 1000200

Academic year 2008/09
1° year of course - Second semester
Professor
Academic discipline
Patologia generale (MED/04)
Field
Discipline mediche e riproduzione umana
Type of training activity
Characterising
10 hours
of face-to-face activities
1 credits
hub:
course unit
in

Integrated course unit module: EXPERIMENTAL MODELS

Learning objectives

Within the scope of the integrated course, the unit aims to provide students with elements and cultural tools for setting up suitable ex vivo and in vitro models for studying physiopathological mechanisms of diseases.

Prerequisites

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Course unit content

<p>The course will develop with classroom lectures that will discuss the following topics: <br />
<br />
Organ and tissue cultures <br />
The extracellular matrix and tissue dissociation <br />
Cell purification <br />
Cell lines <br />
Cloning <br />
Culture of animal cells <br />
Culture contamination <br />
Transfer of gene material <br />
Assessment of cell viability and proliferation <br />
Assessment of cytotoxicity <br />
Organotypical cultures </p>

Full programme

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Bibliography

Reference texts: <br />
<br />
R. Ian Freshney, Culture of Animal Cells: A Manual of Basic Technique, 5th Edition, ISBN: 0-471-45329-3 Ed Wiley- <br />
<br />
Jan-Thorsten Schantz & Kee Woei Ng: A MANUAL FOR PRIMARY HUMAN CELL CULTURE - ISBN 981-238-796-X(pbk) ed. World Scientific <br />
<br />
Alan Doyle , J. Bryan Griffiths: Cell and Tissue Culture: Laboratory Procedures in Biotechnology ISBN: 0-471-98255-5- Ed Wiley <br />
<br />
<br />
<br />
<br />
http://www.protocol-online.org/prot/Cell_Biology/Cell_Culture/ <br />

Teaching methods

TEACHING METHODS: <br />
Classroom lectures with the use of audiovisual aids, discussion in the classroom with the students, individual conversations on request, practical activities in the cell culture laboratory. <br />
<br />
Verification method: <br />
Oral test according to the examination timetable of the Integrated Course. <br />
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Assessment methods and criteria

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Other information

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