Learning objectives
To provide the instruments for the correct management and the macro/micro assessment of tissue samples. To provide the informations and the scientific background to approach correctly the methodology in Clinical Biochemistry.The purpose is a correct use and development of diagnostic products for Clinical Biochemistry and of devices for the collection, treatment and storage of biological samples. To provide the basic knowledge of the principles and methods for the laboratory diagnosis of infectious diseases referring to the main molecular methodologies and technologies related to infections by microrganisms and viruses of medical interest; to deepen the knowledge on the biotechnological applications for the study and the molecular characterization of infectious agents. To provide a theoretical and a practical approach to a correct performance and interpretation of molecular genetics diagnostics
Prerequisites
Exam on Molecular Bases of Infectious Diseases.
Course unit content
Principles of light and electron microscopy; functions and procedures of the Surgical Pathology lab; molecular methods in Surgical Pathology. The biological sample: characteristics, instruction for optimal collection, treatment and conservation (avoiding pre-analytical variation). The main analytical techniques and instrumentations to evaluate the molecules of interest in Clinical Chemistry. Analytical and post-analytical variability affecting the laboratory data. Clinical laboratory quality assurance. Current waived laboratory test. Criteria for test waiving. Kits for diagnostic tests. Evolution Point of care testing (POCT) technology. Quality control in POCT. Principles to detect the markers of organ function and lesion; analytical procedures and clinical correlations. Methodology of therapeutic drug monitoring, detection of toxic compounds, abuse substances and of sport doping. Principles and methods for the laboratory diagnosis of infectious diseases. Biotechnologies in the microbiological and virological diagnosis and for the study and molecular characterization of microrganisms and viruses of medical interest. Molecular methods for detecting susceptibility/resistance of pathogenic agents to chemoantibiotics or anti-viral drugs. Hybridisation Kinetics of nucleic acids. DNA sequencing. Mutationa analysis: DHPLC. HRM. RT-PCR. MLPA. Array CGH.
Full programme
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Bibliography
Kumar, Abbas e Fausto: Robbins e Cotran: Le basi patologiche delle malattie” Ed. Piccin, Padova. R.A. Sacher, R.A. McPherson Interpretazione clinica degli esami di laboratorio Ed. McGraw-Hill, New York. Murray P.R., Baron E.J., Jorgensen J.H., Pfaller M.A., Yolken R.H.'Manual of Clinical Microbiology' Casa Editrice ASM Press; A.M. Molina Romanzi “Microbiologia Clinica. Gli aspetti microbiologici delle malattie infettive" Casa Editrice UTET Periodici; D.O.White, F.J. Fenner Medical Virology Casa Editrice Academic Press; De Carneri "Parassitologia generale ed umana" Casa Editrice Ambrosiana. T.A. Brown Biotecnologie molecolari: Principi e tecniche Zanichelli ed. Bologna 2007.
Teaching methods
Orale lessons and final oral exam.
Assessment methods and criteria
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Other information
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