Learning objectives
<br />
Students must have knowledge of the main methods used in the Pathological Anatomy lab and of their applications.<br />
Moreover, students must demonstrate that they have in-depth knowledge of the molecular biology topics relevant for pathological anatomy purposes and selected according to frequency of use and teaching value:<br />
a) genetics of colorectal familial cancer;<br />
b) pathological anatomy aspects of targeted genetic therapies;<br />
c) role of HPV in cervical cancer pathogenesis and diagnosis.
Prerequisites
Knowledge of basic information in Histology, Anatomy and General Pathology and passing of the relevant examinations
Course unit content
<br />
<br />
Anatomic pathology: from diagnostics to research<br />
Introduction to pathologic anatomy<br />
approach to the morphological study of pathological tissues<br />
Laboratory and techniques in Pathologic Anatomy<br />
Sampling, fixing and processing of the samples<br />
Routine, histomorphological and histochemical colourings!<br />
electronic microscopy procedures<br />
DNA extraction from fixed and embedded tissues <br />
APPLICATONS 1: Approach to familial forms of colo-rectal cancer <br />
FAP and HNPCC: neoplastic hereditary syndromes, the Bethesda panel<br />
Oncogenes, oncosuppressors and the “mismatch repair” system<br />
Microsatellites analysis (LOH and MIN), immunohistochemistry, sequencing.<br />
Search for germinal mutations. <br />
APPLICAZIONI 2: Targeted molecular therapies <br />
Immunohistochemistry and FISH for the research of molecular targets for drugs<br />
APPLICAZIONI 3: HPV and cervical cancer<br />
Search of pathogen agents in histologic samples<br />
The importance of HPV in cervical cancer<br />
In situ hybridization, immunohistochemistry and PCR: detection and characterisation of HPV
Full programme
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Bibliography
<br />
Handbook of the courses given by the teachers<br />
<br />
<br />
Pharoah PD, Caldas C.Molecular genetics and the assessment of human cancers.<br />
Expert Rev Mol Med. 1999 Mar 11;1999:1-19.<br />
<br />
Scarpa A., Ruco L. Anatomia patologica. Le basi. UTET, Torino 2007
Teaching methods
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Assessment methods and criteria
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Other information
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