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University of Parma
Course catalogue
Università degli Studi di Parma
course catalogue

LABORATORY FOR BIOPHYSICS II
cod. 16663

Academic year 2008/09
1° year of course - Second semester
Professor
Academic discipline
Fisica applicata (a beni culturali, ambientali, biologia e medicina) (FIS/07)
Field
Sperimentale-applicativo
Type of training activity
Characterising
72 hours
of face-to-face activities
6 credits
hub:
course unit
in - - -
lectures timetable unavailable
exam calls
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Learning objectives

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Prerequisites

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Course unit content

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A) Methods for separation of macromulecules<br />
Electrophoresis: theory and applications<br />
Chromatography: theory and applications<br />
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B) Fluorescence spectroscopy<br />
Introduction to fluorescence; competitive processes of relaxation; stationary and time resolved fluorescence; spectrofluorimeters; fluorescence intensity and fluorophore concentration: inner filter effect; measure of “quantum yield”; solvent effect; quenching of fluorescence; binding by means of fluorescence; fluorescence polarization and anisotropy: theory and applications; time resolved fluorescence: Time Correlated Single Photon Counting and phase-shift methods.

Full programme

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Bibliography

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<br />
Lakowicz J.R. 'Principles of Fluorescence Spectroscopy' Kluwer Academic/Plenum Publisher (1999) <br />
Cantor C.R. and Schimmel P.R. 'Biophysical Chemistry' W.H.Freeman and Co. N.Y. (1980)

Teaching methods

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Assessment methods and criteria

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Other information

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