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University of Parma
Course catalogue
Università degli Studi di Parma
course catalogue

FUNCTIONAL GENOMICS AND ADVANCED TRANSCRIPTOME ANALYSIS METHODS
cod. 22986

Academic year 2008/09
2° year of course - First semester
Professor
Academic discipline
Biologia applicata (BIO/13)
Field
Discipline biologiche e biochimiche
Type of training activity
Characterising
40 hours
of face-to-face activities
5 credits
hub:
course unit
in - - -
lectures timetable unavailable
exam calls
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Learning objectives

<br />To provide knowledge on the methodologies of functional genomics and their possible applications

Prerequisites

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Course unit content

<br />What is a genome<br />Sequencing genomes<br />How to interpret information in a genomic sequence (in silico methods)<br />Genome anatomy<br />Transcription of complex genomes (human, animal, plant)<br />Synthesis and processing of RNA<br />Regulation of genome activity<br />Analysis of transcriptome<br />Molecular arrays and methods for analysis of arrays<br />Other methods for analysing the transcriptome (differential display, cDNA-AFLP, SAGE)<br />Quantification of the transcriptome<br />Methodologies in RealTime and theoretical bases<br />Genetic-molecular methods to study the function of individual genes (two hybrid, phage display, genetic footprinting, gene inactivation)<br />Site-specific mutagenesis<br />In vivo methods to analyse gene function in higher organisms (plant and animals)<br />Molecular methodologies to obtain knock-in, knock-out animals and mutagenised plants<br />Applications of functional genomics to pharmacology, medicine, agriculture, environment<br /> 

Full programme

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Bibliography

<br />Material provided by the lecturer

Teaching methods

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Assessment methods and criteria

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Other information

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