Learning objectives
This course is aimed to highlight some recent and important applications of genetic and genetic engineering research both in biotechnology and biomedical area. Potentialities and problematic relative to their use will be focused and discussed <br />
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Prerequisites
A good knowledge of genetic and genetic engineering is required
Course unit content
<p> <strong>Genetic engineering and heterologous protein production <br />
The choice of the host <br />
</strong>-Analysis and optimization of the gene expression in prokaryotes. Gene expression from strong and regulatable promoters. Fusion proteins: cleavage and use. Increasing protein stability and secretion. <br />
-Analysis and optimization of the gene expression in eukaryotic microrganisms. Yeast expression vectors, constitutive and regulatable promoters. Secretion of heterologous proteins in S. cerevisiae. Other yeast expression systems: Pichia pastoris e Kluyveromyces lactis. <br />
-Baculovirus- insect cell expression systems. <br />
-Mammalian cell expression systems. Transformation and transfection; alternative DNA delivery methods. Transient and stable expression. Promoters and reporter genes. Viral vectors (SV40, Polioma and papilloma virus).<br />
<strong>The products obtained with the recombinant DNA technology <br />
</strong>-Pharmaceuticals and enzymes. <br />
-Monoclonal antibodies as therapeutic agents.Production of antibodies in microrganisms. <br />
-New vaccines. <br />
-Microbial insecticides <br />
<strong>Protein- protein interactions <br />
</strong>-The yeast two-hybrid system, reverse two hybrid, three hybrids. Phage display<strong>. <br />
-</strong>Genetic methods (soppressori extragenic suppressors, synthetic lethal, mutation gene-dosage dependent)<br />
<strong>Gene therapy <br />
</strong>-Gene transfer protocols <br />
-Ex-vivo and in-vivo gene therapy <br />
-Antisense therapy. <br />
-Applications <br />
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Full programme
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Bibliography
Glick and Pasternak “Biotecnologia molecolare' ed. Zanichelli <br />
Old and Primrose “Ingegneria Genetica' principi e tecniche. Zanichelli <br />
Dale J W. D. and von Schantz 'Dai geni ai genomi'. EdiSES <br />
Teaching methods
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Assessment methods and criteria
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Other information
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