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University of Parma
Course catalogue
Università degli Studi di Parma
course catalogue

BIOMOLECULAR ANALYTICAL CHEMISTRY
cod. 18326

Academic year 2007/08
1° year of course - Second semester
Professor
Academic discipline
Chimica analitica (CHIM/01)
Field
Discipline chimiche
Type of training activity
Basic
24 hours
of face-to-face activities
3 credits
hub:
course unit
in - - -
lectures timetable unavailable
exam calls
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Learning objectives

<br />Introduction to mass spectrometry thecniques and application to biomolecules analysis

Prerequisites

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Course unit content

<br />Separative techniques: Reversed phase Liquid chromatography. Ion exchange liquid chromatography. Capillary electrophoresis. Principles and applications. <br />Mass spectrometry: Low and high resolution Mass analyzer: linear quadrupole (Q), triple quadrupole (QqQ), ion trap (IT), time of flight (TOF), quadrupole-time of flight (QTOF), double focusing sector, Ion cyclotron resonance-fourier transform analyser (FTICR). MS and MSn analysis. Aquisizion signal modes. Qualitative and quantitative analysis. Electrospray: principles and applications of biological interest. <br />Matrix Assisted Laser Desorption Ionization (MALDI)-TOF-MS. Principles and applications.<br />Surface Enhanced Laser Desorption Ionization (SELDI)-TOF-MS. Principles and applications.<br />Inductively coupled plasma (ICP)-MS. Sample preparation methods for the mass spec analysis. Hyphenated techniques: liquid chromatography-mass spectrometry. Capillary electrophoresis-mass spectrometry. Liquid chromatography-ICP-MS.<br />Analytical data quality: evaluation of analytical data reliability in mass spectrometry.

Full programme

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Bibliography

<br />C. Dass Principles and practice of biological mass spectrometry. Ed. Wiley.

Teaching methods

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Assessment methods and criteria

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Other information

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