Vai al contenuto principale Vai al footer
Università degli Studi di Parma Università degli Studi di Parma
University of Parma
Course catalogue
Università degli Studi di Parma
course catalogue

PROTEIN ENGINEERING
cod. 18331

Academic year 2007/08
1° year of course - Second semester
Professor
Academic discipline
Biologia molecolare (BIO/11)
Field
Discipline biologiche e biologiche applicate
Type of training activity
Characterising
24 hours
of face-to-face activities
3 credits
hub:
course unit
in - - -
lectures timetable unavailable
exam calls
Print

Learning objectives

The course aims to introduce the advanced Molecular Biology techniques used for isolating eukaryotic genes and studying their expression, and for producing and modifying &nbs p;recombinant proteins in the E.coli bacterium.

Prerequisites

- - -

Course unit content

<br />- Isolation of a specific gene (cDNA, genome region that contains it). <br />
<br />
- Transcriptional analysis of the gene whose cDNA has been isolated. <br />
<br />
- Analysis of the structure of the genome region containing the gene of interest. <br />
<br />
- Expression in bacteria of the protein coded by the gene. <br />
<br />
- Mutagenesis of the gene and of the recombinant protein coded by it. <br />
<br />
- Chemical synthesis of DNA and creation of artificial genes. 

Full programme

- - -

Bibliography

Analisi dei geni e genomi - Richard J.Reece - EdiSES <br />
Ingegneria genetica. Principi e tecniche. S.Primrose et al. - Zanichelli <br />
DNA Ricombinante - J.D.Watson et al. - Zanichelli

Teaching methods

- - -

Assessment methods and criteria

- - -

Other information

- - -