Learning objectives
Aims of the course is the study of genetic approaches applied to genomics and functional analysis in eukaryotes model organisms. Particular attentions will be payed to model organisms in fundamental and biotechnological applications. In addition, the course will study molecular aspects of human genetics and application to medical problems.
Prerequisites
None
Course unit content
The course consits of two sections:
Molecular genetics of model organisms
Yeast as model organism
Functional analysis in plants
C.elegans as animal model
Drosophila melanogaster as animal model
Zebra fish as vertebrate model
Human molecular genetics
Pharmacogenetics.
Gene therapy
Forensic genetics
Full programme
Molecular genetics of model organisms
YEAST AS MODEL ORGANISM
Forward e reverse genetics- Genome sequencing in S. cerevisiae- Comparative genomics in yeasts- S. cerevisiae deletant collection and use of null mutants in functional analysis- Methods for gene interaction studies applied to human diseases - Yest as model organism for the study of mitochondrial genetics- Yeast as model organism for the study of mitochondrial diseases.
FUNCTIONAL ANALYSIS IN PLANTS
Gene silencing
RNA interference in plants
MicroRNA
C.elegans AS ANIMAL MODEL
Forward e reverse genetics in C. elegans. Functional analysis by RNAi - C. elegans as model organism for the study of human pathologies.
Drosophila melanogaster AS ANIMAL MODEL
Forward e reverse genetics in D. melanogaster. - D. melanogaster as model organism for the study of human pathologies.
Zebra fish as vertebrate model
Forward e reverse genetics in Zebra fish. Morpholino tecnique. Knok out of genes by Zn finger nucleases, TALENs and CRISPRs-Cas9. Zebra fish as model organism for the study of human pathologies.
Human molecular genetics
Pharmacogenetics.
Gene therapy
Forensic genetics
Bibliography
Philip Meneely
Analisi Genetica Avanzata
ED. McGraw-Hill
T. Strachan, A.P.Read
Genetica molecolare umana
Zanichelli
M.Giacca
Terapia genica
Springer Biomed 2014
Ricci, Previderè, Fattorini, Corradi
La prova del DNA per la ricerca della verità
Giuffrè Ed
Original papers suggested by the teacher.
Teaching methods
Frontal lectures and exercises
Assessment methods and criteria
Final written and oral examination
Other information
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