Learning objectives
Laboratorio di Fisiologia (SSD BIO/09) 4 CFU su 20<br />(Responsabile: Donatella Stilli)<br /><br /><br /> to provide basic knowledge on the appropriateexperimentalapproaches for the study of biological functions atdifferent levels ofcomplexity, i.e. from intact organism to molecularlevel <br />
Prerequisites
<br />- Biochemistry<br />- Molecular biology<br />- General physiology<br />- Cell Biology<br /><br /><br /><br />
Course unit content
<br /> <br /><br />Physiology lab. (SSD BIO/09) 4 CFU <br />( Donatella Stilli)<br /><br /><br />-Hemodynamicmeasurementsfrom anaesthetized animals: arterial bloodpressure,leftventricular systolic andend-diastolicpressures anestetizzato, max dP/dt e min dP/dT<br /><br />- Processing and statistical treatment of hemodynamic data.<br /><br />- Surgery on small animals: chronic implant of miniaturized transmitters for telemetry ECG recordings, in the rat. <br /><br />- Telemetry ECG recording and processing<br />- Enzymatic isolation of cardiomyocytes from rat hearts. <br />- Measurements of cellular electrophysiological properties in isolated cardiomyocytes by the patch-clamp technique. <br /><br />- Morphometrical analysis of the myocardial ventricular tissue, from normal or pathological rat hearts.<br /> <br /> <br /> <br /><br /><br />
Full programme
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Bibliography
provided by the teacher<br />
Teaching methods
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Assessment methods and criteria
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Other information
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