Learning objectives
<p>Objectives of the course : </p>
<p>To provide the second year students with the basic concepts of classical genetics examples of genetic strategies to answer to specific questions. <br />
Each topic will be presented in experimental form, with an attempt to answer to the following questions: what was the reason for the experiment, how was it carried out, what result did it lead to and what is the meaning of the results? <br />
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Prerequisites
First year Genetics
Course unit content
General regulatory systems.- Glucose repression in bacteria: physiological analysis and selection of mutants - Control of glucose repression in bacteria: cAMP and CAP- Glucose repression in yeast. Physiological analysis - Genetic control of glucose repression- Strategies to select glucose non-represseible and non-derepressible mutants- Genetic analysis of the selected mutants- Physiological analysis and determination of glucose uptake- Headie-Hofstee plot of the mutants: Identification of low and high affinity transporters- Reversion and suppression. - Search of suppressors to find out new genes. - Petite mutants in yeast. - Genetic and physiological analysis. Non- Mendelian inheritance.
Full programme
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Bibliography
Papers related to the specific subjects will be indicated to the students attending to the course
Teaching methods
Teaching methods: Oral and practical lesson <br />
Assessment methods: written exam <br />
Language of instruction: Italiano <br />
Assessment methods and criteria
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Other information
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