Learning objectives
This course will offer an overview o the methodologies based on the fluorescence emission by organic molecules useful for the characterization of functional a structural properties of biological macromolecules<br />
The aim of this course is to introduce qualitatively experimental methodologies and discuss their potential and their applicability.<br />
Laboratory experiences will allow students to apply directly the concepts outlined during the introductory part.
Prerequisites
Mathematics and Physics (laurea triennale). Basic concepts of optical absorbance spectroscopy.<br />
Basic knowledge of photophysical processes in organic molecules
Course unit content
Introduction<br />
Molecular photophysical processes<br />
Radiative processes<br />
Non radiative processes<br />
Intermolecular interactions<br />
Jablonski diagram<br />
Fluorescence<br />
Lifetime<br />
Quantum yield<br />
Decay kinetics<br />
Excitation and emissione spctra<br />
Solvent effect on the emission spectra<br />
Polarization<br />
Interactions with solutes<br />
FRET<br />
Steady state and time resolved methods<br />
<br />
<strong>Laboratory</strong><br />
Excitation and emission spctra of organic compounds<br />
Fluorescence quenching and intermolecular interactions (Stern Volmer)<br />
Fluorescence emission by aromatic amino acids<br />
FRET inside proteins<br />
Fluorescence emission by labeled proteins<br />
Emission spectra by Green fluorescent protein (GFP)<br />
Binding of organic dyes to proteins
Full programme
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Bibliography
La luminescenza. Elementi di fotofisica molecolare<br />
Ivan Baraldi<br />
Bononia University Press
Teaching methods
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Assessment methods and criteria
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Other information
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