Vai al contenuto principale Vai al footer
Università degli Studi di Parma Università degli Studi di Parma
Degree course in
Biotechnology
Università degli Studi di Parma
Department of Chemistry, Life Sciences and Environmental Sustainability

RECOMBINANT TECHNOLOGIES AND INTEGRATED BIOTECHNOLOGIES LABORATORY II
cod. 1004368

Academic year 2011/12
2° year of course - Second semester
Professor
Academic discipline
Biologia applicata (BIO/13)
Field
Discipline biotecnologiche con finalità specifiche: biologiche e industriali
Type of training activity
Characterising
90 hours
of face-to-face activities
9 credits
hub: PARMA
course unit
in - - -
lectures timetable unavailable
exam calls
Print

Learning objectives

to provide theoretical and practical knowledge about the main methodologies for gene analysis in microorganisms, animals and plants

Prerequisites

- - -

Course unit content

1. Basic module - Fundamental principles of gene cloning - Importance of gene cloning - Cloning vectors: plasmids and bacteriophages - Methods for DNA purification - Methods for DNA manipulation - How to introduce DNA in living cells; transformation and selection of ricombinant clones - Cloning vectors for bacteria different from E. coli - Cloning vectors for fungi and yeasts - How to select a specific gene and analyse a cloned gene
2.Cloning in plants - Cloning vectors for plant organisms - Transformation methods for plant organisms - Obtainment of transgenic plants
3. Cloning in animal cells - Growth and properties of animal cells in culture - Selection systems for animal cells - Gene transfer into animal cells - Plasmid vectors for animal cells, amplicons and expression vectors - The fate of transferred DNA - Viral vectors and retroviral vectors for animal cells
4. Transgenic animals - Gene transfer into animals - Gene manipulation in animals - Transgenic animals - Manipulation of embryo and somatic cells
5. Gene therapy - Principles and methods - Experimental models - Some experimental results - Potential human models

Full programme

- - -

Bibliography

"Gene cloning an introduction" T.A. Brown (1986) Van Nostrand Reinhold (UK)

"Ingegneria Genetica" SM Kingsman, AJ Kingsman (1988) Piccin, Padova

"Biotecnologia molecolare" BR Glick, JJ Pasternak (1999) Zanichelli, Bologna

"Genetica generale e umana" volume secondo, L De Carli, E Boncinelli, GA Danieli, L Larizza (1998) Piccin, Padova

Teaching methods

Oral lesson and laboratory

Assessment methods and criteria

written and oral examination

Other information

- - -