CLINICAL BIOCHEMISTRY DIAGNOSTIC TECHNIQUES
cod. 12736

Academic year 2012/13
3° year of course - Second semester
Professor
Academic discipline
Scienze tecniche di medicina di laboratorio (MED/46)
Field
Diagnostica di laboratorio biomedico
Type of training activity
Characterising
80 hours
of face-to-face activities
4 credits
hub:
course unit
in - - -

Learning objectives

The aim of this course is to provide the cultural basis for understanding the basic mechanisms both of the techniques commonly employed in the laboratory of clinical biochemistry (routine analysis) and some examples of more advanced test, found only in specialized laboratories.

Prerequisites

Besides the obligatory prerequisites stated by the course of degree, Clinical Biochemistry is an interdisciplinary science and needs the basic knowledge in Chemistry, Biochemistry and Physiology.

Course unit content

Analysis by the clinical laboratory: definition, typology, request.

Biological samples: collection, treatment and storage.

Instrument calibration; internal quality control; external quality control. Evaluation of the analytical performance.


Methods in the quantitative analysis of markers (function or damage) of liver, kidney, heart and skeletal muscle.

Urinalysis. Proteinuria and micro-proteinuria.

Analysis of plasma and urinary proteins: nephelometry, electrophoresis and immuno-fixation, capillary electrophoresis.

Enzyme determination in clinical chemistry. Activity or mass assay.

Lipids and dislipoproteinemia: principles and methods of analysis

Carbohydrates: methods for glycemia quantification.

Hormone assays.


The clinical laboratory in the autoimmune diagnostics. Immuno-enzymatic approach; principles and measure of a fluoroscopic preparation.

The clinical laboratory in the allergologic diagnostics.

Full programme

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Bibliography

A.Burlina. Medicina di laboratorio. Fondamenti di diagnostica -C.G. Edizioni medico scientifiche S.R.L.

Teaching methods

Front lessons and laboratory practice

Assessment methods and criteria

Oral examination

Other information

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