Learning objectives
To acquire the theoretical knowledge needed to understand and put into practice the basic techniques to be used in the Pathological Anatomy laboratories, including, among the others: tissue processing for histopatology, sectioning and staining (histochemical, immunohistochemical) of histological slides, application of molecular biology techniques to the study of pathological processes
Prerequisites
Knowledge of the basis of histology.
Course unit content
The course will be mainly focused on the morphologic and molecular techniques applied to the study of pathologic tissues
Full programme
Organization of the histopathology laboratory
Tissue sampling, fixation and processing
Intraoperative frozen sections examination and cryotomy
Cytodiagnostic techniques
Cell cultures
Molecular biology techniques applied to Pathologic Anatomy (Polymerase Chain Reaction, Fluorescent In Situ Hybridization
- Theoretical basis of FISH technology
- FISH applications to the study of numerical and structural chromosomal aberrations
- FISH analysis implications to oncologic treatment with targeted-therapies
- Set-up of a FISH experiment in the laboratory.
Bibliography
AA.VV. Anatomia Patologica: le basi. A cura di L. Ruco, A. Scarpa. UTET Scienze Mediche, Torino, 2007
AA.VV. Manuale di Tecnica Cito-Istologica. A cura di S. Daniel, T. Zanin.
Documentazione Scientifica Editrice (DSE), Bologna, 1997
Printed forms of the Power Point slides presented during the course
Teaching methods
Frontal teaching.
Assessment methods and criteria
Final oral exam.
Other information
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